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利用纳米金刚石对多种分子具有较强吸附能力及团聚时特殊的空间构型,依次将异硫氰酸荧光素(FITC)、10-羟基喜树碱(HCPT)及转铁蛋白(TF),负载于纳米金刚石(NDs)上,形成复合物,使NDs成为具有多种功能的药物载体。使用透射电镜、红外光谱仪、紫外分光光度仪等对NDs及其复合物进行表征及分析,用荧光显微镜及细胞存活率检测方法观察,并检测了复合物的细胞效应。结果表明,HCPT在NDs上3天的吸附量为42.5%;存活率结果显示,复合物组的细胞存活率明显低于单独HCPT组,TF-HCPT-ND复合物组的存活率最低;荧光显微镜下,观察荧光标记的复合物孵育Hela细胞3h后,TF-HCPT-ND组的荧光强度明显高于HCPT-ND组,这与TF-HCPT-ND组的细胞存活率最低的结果相符。本文为构建纳米金刚石的多功能药物靶向输运系统提供了基础。
Using nanodiamonds, which have strong adsorption capacity for many kinds of molecules and special spatial configuration during reunion, FITC, HCPT and TF, Load on the nano-diamond (NDs), the formation of complexes, NDs become a multi-functional drug carrier. The characteristics of NDs and their complexes were characterized and analyzed by transmission electron microscopy, infrared spectroscopy and UV spectrophotometer. Fluorescence microscopy and cell viability assay were used to observe the cellular effects of the complexes. The results showed that the amount of HCPT adsorbed on NDs for 3 days was 42.5%. The survival rate showed that the cell viability of the composite group was significantly lower than that of the HCPT group alone, and the survival rate of the TF-HCPT-ND complex group was the lowest. The fluorescence microscopy , The fluorescence intensity of TF-HCPT-ND group was significantly higher than that of HCPT-ND group after 3h incubation with fluorescent labeled complex, which was consistent with the lowest cell viability in TF-HCPT-ND group. This article provides a basis for the construction of a multi-functional drug targeting delivery system for nanodiamonds.