目的观察噪声对幼鼠学习记忆及海马单胺类神经递质的影响,探讨噪声对幼鼠学习记忆的影响机制。方法将24只SD幼鼠,随机分为噪声组和对照组,噪声组在80 dB(A)噪声下持续暴露1个月,用Morris水迷宫测试幼鼠学习记忆能力,用高效液相色谱-电化学法检测幼鼠海马单胺类神经递质多巴胺(DA)、去甲肾上腺素(NE)和5-羟色胺(5-HT)的含量。结果 Morris水迷宫定航实验噪声组幼鼠4 d的平均逃避潜伏期分别为64.54 s、31.98s、14.69 s、6.95 s,均较对照组明显延长(P<0.05);空间搜索实验噪声组幼鼠跨台次数为6.75次、距离百分比为25.19%、时间百分比为23.90%,均较对照组明显降低(P<0.01)。高效液相色谱-电化学法检测发现噪声组幼鼠海马DA、NE和5-HT的含量分别为253.18 ng/g、140.01 ng/g、114.21 ng/g,均较对照组明显减少(P<0.01)。结论噪声暴露下幼鼠海马DA、NE、5-HT含量降低,幼鼠学习记忆能力下降。 Objective To observe the effect of noise on learning and memory and monoamine neurotransmitter in hippocampus of young rats and explore the mechanism of noise on learning and memory in young rats. Methods Twenty-four SD rats were randomly divided into noise group and control group. The noise group was exposed to 80 dB (A) noise for 1 month. Morris water maze test was used to study the learning and memory ability of juvenile rats. High performance liquid chromatography- The levels of monoamine neurotransmitters such as dopamine (DA), norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in the hippocampus of young rats were detected by electrochemical method. Results The mean escape latency of 4-day-old pups in Morris water maze test was 64.54 s, 31.98 s, 14.69 s and 6.95 s, respectively, which were significantly longer than those in control group (P <0.05) The number of cross-platform was 6.75 times, the distance percentage was 25.19% and the time percentage was 23.90%, which were significantly lower than those in the control group (P <0.01). The contents of DA, NE and 5-HT in the hippocampus of noise-induced neonatal rats were 253.18 ng / g, 140.01 ng / g and 114.21 ng / g, respectively, which were significantly lower than those of the control group by high performance liquid chromatography-electrochemical detection (P < 0.01). Conclusion The levels of DA, NE and 5-HT in hippocampus of young rats are decreased after noise exposure, and the learning and memory abilities of young rats are decreased.