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研究马尾松花粉多糖硫酸酯化前后对小鼠脾细胞内游离钙离子的影响及作用机理。应用氯磺酸-吡啶法将马尾松花粉多糖进行硫酸化修饰,采用钙离子荧光探针Fura 2-am测定修饰前后的两种多糖对小鼠脾细胞内游离钙离子浓度。马尾松花粉多糖经硫酸酯化后可显著提高脾细胞内钙离子浓度,而酯化前作用不明显。特异性L型钙通道阻滞剂维拉帕米可部分抑制酯化多糖促钙离子浓度升高的作用。在外钙为零时,酯化多糖仍可小幅度提高脾细胞钙离子浓度。马尾松花粉多糖经硫酸化修饰后可通过引发外钙内流和内钙释放两条通路提高脾细胞钙离子浓度。
To study the effect and mechanism of intracellular free calcium in mouse spleen cells before and after the sulfation of masson pine pollen polysaccharide. Chlorosulfonic acid-pyridine method was used to sulfonate the pollen polysaccharide of Masson pine and the calcium ion fluorescence probe Fura 2-am was used to measure the concentration of free calcium in mouse spleen cells. Masson pollen polysaccharide sulfation can significantly increase the intracellular calcium ion concentration, while the role of esterification is not obvious. Verapamil, a specific L-type calcium channel blocker, can partially inhibit the increase of calcium ion concentration of esterified polysaccharides. When the external calcium is zero, esterified polysaccharide can still slightly increase the concentration of calcium in spleen cells. After the sulfated modification of Masson Pine Pollen Polysaccharide, the calcium concentration in spleen cells can be increased by initiating two pathways of Ca2 + influx and Ca2 + release.