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目的:观察自发性高血压大鼠(SHR)心肌的血管紧张素转化酶(ACE)和ACE2的表达,以探讨ACE2和ACE在高血压发生发展中的变化。方法:取15只SHR,处死,分离左心室,行RT-PCR、Western blot蛋白质免疫印迹和免疫组织化学检测ACE及ACE2表达;同步取10只WKY大鼠作为正常血压对照组。结果:SHR组心肌ACE的mRNA和蛋白质表达都显著高于WKY组[(1.68±0.34)∶(0.33±0.12),P<0.05;(1.21±0.14)∶(0.71±0.11),P<0.05],而ACE2的mRNA和蛋白质表达皆明显低于WKY组[(0.50±0.15)∶(1.16±0.24),P<0.05;(0.71±0.24)∶(1.22±0.14),P<0.05)]。免疫组织化学染色显示,SHR组ACE的阳性率明显高于WKY组(87%∶50%,P<0.05),而ACE2的阳性率明显低于WKY组(27%∶70%,P<0.05)。结论:SHR心肌ACE明显升高,ACE2显著降低;SHR高血压发生发展过程中存在着ACE和ACE2表达的失衡。
Objective: To investigate the expression of angiotensin-converting enzyme (ACE) and ACE2 in spontaneously hypertensive rats (SHR) myocardium in order to investigate the changes of ACE2 and ACE in the development of hypertension. METHODS: Fifteen SHRs were isolated and the left ventricle was sacrificed. The expressions of ACE and ACE2 were detected by Western blotting and immunohistochemistry. The WKY rats were taken as normal control group. Results: The mRNA and protein expression of ACE in SHR group were significantly higher than those in WKY group [(1.68 ± 0.34) vs (0.33 ± 0.12), P <0.05; (1.21 ± 0.14) vs (0.71 ± 0.11), P <0.05] , While ACE2 mRNA and protein expression were significantly lower than the WKY group [(0.50 ± 0.15) :( 1.16 ± 0.24, P <0.05; (0.71 ± 0.24): (1.22 ± 0.14), P <0.05)]. Immunohistochemical staining showed that the positive rate of ACE in SHR group was significantly higher than that in WKY group (87%, 50%, P <0.05), but the positive rate of ACE2 in SHR group was significantly lower than that in WKY group (27%, 70%, P <0.05) . CONCLUSIONS: The myocardial ACE in SHR is significantly increased and ACE2 is significantly decreased. The imbalance of ACE and ACE2 expression exists in the development of SHR.