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本法用10%的三氯乙酸快速提取食物样品中的生物胺,提取物经离子交换柱纯化后用邻苯二醛(OPA)/2-巯基乙醇进行柱前衍生,生成胺-OPA 加合物,经反向高效液相色谱(RP-HPLC)分离后,用荧光检测器检测。
This method uses 10% trichloroacetic acid rapid extraction of biogenic amines in food samples, the extract was purified by ion exchange column with orthophthalaldehyde (OPA) / 2-mercaptoethanol for pre-column derivatization to generate amine-OPA adduct The material was separated by reverse-phase high-performance liquid chromatography (RP-HPLC) and detected with a fluorescence detector.