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目的观察他克莫司对朗格汉斯细胞（ LC）迁移能力的影响，为明确他克莫司在治疗疾病中的作用途径打下基础。方法在不同时间段内将不同浓度的他克莫司腹腔注射 C57BL/6小鼠，再用异硫氰酸荧光素 (FITC)刺激小鼠耳背部皮肤， 12 h后通过流式细胞仪检测小鼠耳背部淋巴结内摄取抗原的 LC数量。通过免疫组化法检测抗原致敏处 LC的数量。结果他克莫司腹腔注射过的小鼠耳背皮肤受到 FITC刺激后，其皮肤局部摄取抗原的 LC迁移至局部淋巴结的数量明显减少，尤其在 FITC刺激前 12 h注射他克莫司的小鼠皮肤局部 LC的迁移能力受到显著抑制，在注射高剂量他克莫司组与低剂量组之间存在明显差异。而同时他克莫司腹腔注射过的小鼠，其耳背部受到 FITC刺激后的皮肤局部 LC的数量高于未注射组。结论他克莫司作为一种免疫抑制剂，其发挥治疗作用途径有可能是通过抑制 LC的迁移能力，从而阻止或减弱了某些免疫反应。
Objective To observe the influence of tacrolimus on the migration of Langerhans cells (LC), and lay a foundation for clarifying the role of tacrolimus in the treatment of diseases. Methods C57BL / 6 mice were injected intraperitoneally with different concentrations of tacrolimus in different time periods, and then stimulated with FITC to stimulate the skin of the ears of the mice. After 12 hours, the cells were detected by flow cytometry The number of LCs for antigen retrieval in the dorsal rachis of mouse ears. Immunohistochemistry was used to detect the number of LC allergens. Results The number of local LC-induced antigen LC migration to the local lymph nodes was significantly decreased after FITC stimulation in the dorsal skin of mice injected intraperitoneally with tacrolimus, especially in mice injected with tacrolimus at 12 h before FITC stimulation Local LC migration ability was significantly inhibited, there was a significant difference between high-dose injection of tacrolimus and low-dose group. While the mice injected with tacrolimus intraperitoneally had a higher number of LC in the skin than the non-injected mice after FITC stimulation. Conclusion Tacrolimus, as an immunosuppressive agent, may play a therapeutic role in preventing and attenuating certain immune responses by inhibiting LC migration.