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目的:探讨人端粒酶逆转录酶(hTERT)mRNA在宫颈癌(CC)脱落细胞中的表达及其与临床病理参数之间的关系。方法:采用实时荧光定量RT-PCR技术,检测人宫颈癌HeLa细胞株(细胞组)、40例宫颈癌(宫颈癌组)和21例正常或慢性宫颈炎(对照组)患者宫颈脱落细胞中hTERT mRNA的表达,以细胞组hTERTmRNA的表达作为定性、定量参照。结果:宫颈癌组和对照组中hTERT mRNA阳性表达率分别为82.5%和9.5%,差异有统计学意义,P=0.000;两组hTERTmRNA的相对定量水平NhTERT分别为0.327 6~13.782 3和0.009 3~0.081 6,差异均有统计学意义,P=0.019;hTERTmRNA阳性表达与宫颈癌的分化程度具有负相关性(r=-0.308,P=0.016),与年龄、临床分期、间质浸润深度和组织学分类无相关性。结论:宫颈癌患者宫颈脱落细胞hTERT mRNA阳性表达率及相对定量水平均异常增高,检测宫颈脱落细胞hTERT mRNA的表达有助于宫颈癌的诊断及预后评估。
Objective: To investigate the expression of human telomerase reverse transcriptase (hTERT) mRNA in cervical cancer (CC) exfoliated cells and its relationship with clinicopathological parameters. Methods: Real-time fluorescent quantitative RT-PCR was used to detect hTERT in cervical exfoliated cells of HeLa cell lines (group of cells), 40 cases of cervical cancer (cervical cancer group) and 21 cases of normal or chronic cervicitis (control group) mRNA expression, hTERT mRNA expression in the cell group as a qualitative and quantitative reference. Results: The positive expression rates of hTERT mRNA in cervical cancer group and control group were 82.5% and 9.5% respectively, with statistical significance (P = 0.000). The relative quantitative levels of hTERT mRNA in the two groups were 0.327 6-13.782 3 and 0.009 3 ~ 0.081 6, P = 0.019; The positive correlation between hTERT mRNA expression and the degree of cervical cancer was negatively correlated (r = -0.308, P = 0.016), with age, clinical stage, Histological classification no correlation. Conclusion: The positive rates and the relative quantitative levels of hTERT mRNA in cervical exfoliated cells of patients with cervical cancer are abnormally elevated. Detecting the expression of hTERT mRNA in cervical exfoliated cells is helpful for the diagnosis and prognosis evaluation of cervical cancer.