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目的 探讨雌、孕激素对体外培养人类子宫内膜腺上皮细胞降钙素（Ｃｔ）表达的影响及其意义。方法 建立子宫内膜 腺上皮细胞体外培养体系．分别用雌二醇（Ｅ２）、孕酮（Ｐ４）刺激细胞２４ｈ、４８ｈ，其浓度分别为１× １０－７、１×１０－８、１×１０－９ｍｏｌ／Ｌ 和１×１０－６、１×１０－７、１×１０－８ｍｏｌ／Ｌ，利用流式细胞仪对Ｃｔ表达阳性细胞进行检测。结果 性激素影响体外培养的人子宫内膜 上皮细胞Ｃｔ的表达，Ｐ４促进Ｃｔ表达，并呈剂量依赖性，１×１０－６ｍｏｌ／Ｌ作用２４ｈ，其促进效应最强；而低剂量Ｅ２对Ｃｔ表 达无明显影响，高浓度Ｅ２则抑制其表达。结论 子宫内膜上皮细胞Ｃｔ的表达受性激素调节，在人类胚胎着床过程中起 重要作用。
Objective To investigate the effect of estrogen and progesterone on the expression of calcitonin (Ct) in cultured human endometrial glandular epithelial cells and its significance. Methods To establish the in vitro culture system of endometrial glandular epithelial cells. The cells were stimulated with estradiol (E2) and progesterone (P4) for 24 h and 48 h, respectively. The concentrations were 1 × 10-7, 1 × 10-8, 1 × 10-9 mol / L and 1 × 10-6, 1 × 10-7,1 × 10-8mol / L, the use of flow cytometry Ct positive cells were detected. Results Sex hormones affected the expression of Ct in human endometrial epithelial cells cultured in vitro, and P4 promoted the expression of Ct in a dose-dependent manner. The effect of 1 × 10-6 mol / L for 24h was the strongest, while the effect of low-dose E2 on Ct No significant effect, high concentration of E2 inhibits its expression. Conclusion The expression of Ct in endometrial epithelial cells is regulated by sex hormones and plays an important role in the implantation of human embryo.