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目的探索单极高频电凝对小鼠阴茎、肾脏、睾丸损伤的病理实验研究。方法依据电凝方式(W)/电凝持续时间(s)/电凝间隔时间(s)的不同,将100只KM小鼠随机分为7组:组1-20W/0.5s/0.5s;组2-40W/0.5s/0.5s;组3-20W/1.0s/1.0s;组4-30W/0.5s/2.0s;组5-30W/1.0s/1.0s;组6-40W/0.5s/2.0s;组7为空白组。对除组7外的六组进行单极高频电凝对小鼠阴茎、肾脏、睾丸损伤的病理实验研究。空白组不做电凝处理,直接取上述三脏器做病理检查。实验组每组16只,其中电凝后即刻处死4只,3d后处死8只,15d后处死4只。结果组1、组2较组4、组6坏死面积更大,差异有统计学意义(P均<0.01);其中组2和组6相比较差异有统计学意义(P<0.01);组3、组5电凝后坏死面积明显大于实验组其他四组(P<0.01),组5大于组3,差异有统计学意义(P<0.05)。结论在使用高频电刀电凝时,持续时间过长,间隔时间过短,功率过大都是加重肾脏损伤的重要因素;高频电刀对肾脏的损伤重于对阴茎和睾丸的损伤。
Objective To explore the pathological experimental study on the damage of penile, kidney and testis in mice by unipolar high frequency electrocoagulation. Methods 100 KM mice were randomly divided into 7 groups according to the method of electrocoagulation (W) / coagulation duration (s) / coagulation interval (s): group 1-20W / 0.5s / 0.5s; Group 2-40W / 0.5s / 0.5s; group 3-20W / 1.0s / 1.0s; group 4-30W / 0.5s / 2.0s; group 5-30W / 1.0s / 1.0s; group 6-40W / 0.5 s / 2.0s; group 7 is a blank group. The histopathological study on the penile, kidney and testis lesions of six groups except group 7 under monopolar high frequency electrocoagulation. Blank group without coagulation treatment, direct access to the above three organs do pathological examination. Sixteen rats in each group were sacrificed in the experimental group, including 4 immediately after electrocoagulation, 8 after 3 days and 4 after 15 days. Results Group 1, group 2 compared with group 4, group 6 necrosis larger, the difference was statistically significant (P <0.01); Group 2 and 6 compared with the difference was statistically significant (P <0.01); Group 3 , And the area of necrosis after electrocoagulation in group 5 was significantly larger than that in the other four groups (P <0.01). The number of necrosis in group 5 was greater than that in group 3 (P <0.05). Conclusion When using high-frequency electrocautery coagulation, the duration is too long, the interval is too short, the power is an important factor aggravating the kidney injury; high-frequency electric knife on the kidney more than the damage to the penis and testicles.