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目的研究伊拉地平对鱼藤酮处理的神经母细胞瘤(SH-SY5Y)细胞的保护作用,以探讨伊拉地平对帕金森病的治疗作用及可能的机制。方法分为4组①对照组(DMSO处理);②伊拉地平组(伊拉地平250 nmol.L-1干预);③鱼藤酮组(鱼藤酮200 nmol.L-1干预);④鱼藤酮+伊拉地平组(鱼藤酮200 nmol.L-1+伊拉地平250 nmol.L-1干预);每组5个复孔。于伊拉地平、鱼藤酮、鱼藤酮+伊拉地平、DMSO分别干预SH-SY5Y细胞2 h后,用CCK-8法检测细胞存活率,观察线粒体膜电位、细胞内Ca2+变化、LC3表达和透射电镜观察细胞自噬情况。结果鱼藤酮组细胞存活率下降,自噬增多;鱼藤酮+伊拉地平组存活率相对下降幅度小,自噬体形成少。结论伊拉地平具有减轻由鱼藤酮引起的SH-SY5Y细胞的自噬现象。
Objective To study the protective effect of isradipine on rotenone-treated neuroblastoma (SH-SY5Y) cells in order to investigate the therapeutic effect and possible mechanism of isradipine on Parkinson’s disease. The rats were randomly divided into 4 groups: ① control group (DMSO treatment); ② ilapidipine group (250 nmol.L-1 intervention); ③ rotenone group (rotenone 200 nmol.L-1 intervention) Hepatic group (rotenone 200 nmol.L-1 + Irala level 250 nmol.L-1 intervention); each group of 5 complex hole. The survival rate of SH-SY5Y cells was detected by the method of CCK-8. The changes of mitochondrial membrane potential, intracellular Ca2 +, LC3 expression and transmission electron microscopy were observed in the SH-SY5Y cells treated with ilapirtime, rotenone, rotenone + Cell autophagy. Results The survival rate of rotenone group was decreased and the autophagy was increased. The survival rate of rotenone + andlamedipine group decreased relatively less and the formation of autophagosome was less. Conclusion Ilapirtine can reduce the autophagy of SH-SY5Y cells induced by rotenone.