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为了探明低磷胁迫对烟草糖代谢及养分吸收的影响机理。以云烟87为试验材料,设置正常供磷(T1,1 mmol/L Pi)和低磷(T2,0.1 mmol/L Pi)两个处理的沙培试验,检测低磷胁迫下烟株不同部位的蔗糖、淀粉、营养元素的含量及糖代谢相关酶的活性,分析糖代谢关键基因(SUT1、INV、AGPase)及高亲和营养元素转运蛋白家族基因(PT1、PT2、HAK1、IRT1)的表达差异。结果表明:①低磷胁迫显著增加了烟株根部和地上部的蔗糖含量;虽然焦磷酸化酶(AGPase)基因在低磷条件下表达量下调,但是由于淀粉酶活性被强烈抑制,淀粉含量仍显著增加;②低磷胁迫增加了烟株根部和地上部K和Fe的含量,降低了Mg的含量,Ca含量差异不显著;K和Fe的积累可能是由高亲和钾转运蛋白(HAK1)及铁调控转运体基因(IRT1)的表达量提高引起的,为磷与其他营养元素之间的互作提供理论基础。
In order to explore the mechanism of low phosphorus stress on tobacco sugar metabolism and nutrient uptake mechanism. Yunfu 87 was used as experimental material to set up sand culture experiment of two treatments of normal phosphorus supply (T1, 1 mmol / L Pi) and low phosphorus (T2,0.1 mmol / L Pi) (PT1, PT2, HAK1, IRT1) of key genes of glucose metabolism (SUT1, INV, AGPase) and high affinity nutrient transporter family of genes (PT1, PT2, HAK1, IRT1) . The results showed that: (1) Low-P stress significantly increased the sucrose content of roots and shoots of tobacco plants. Although the expression of AGPase gene was down-regulated under low phosphorus conditions, the starch content was still high due to the strong inhibition of amylase activity (P <0.05); (2) Under low P stress, the content of K and Fe in root and shoot of tobacco increased, the content of Mg decreased, but the content of Ca was not significant. The accumulation of K and Fe might be caused by the high affinity potassium transporter (HAK1) (IRT1) gene expression and provide the theoretical basis for the interaction between phosphorus and other nutrients.