原生质体融合是一个由选择亲本,原生质体制备、融合、再生、建立标记性状,融合子挑选和验证等各个环节组成的技术体系.在这个体系中,融合子(fusion product)的遗传验证是一个最为重要的步骤,即便是以营养缺陷型或抗药性突变体做为标记,也只是为挑选融合子提供了一个可靠的依据,并不能代表融合子的遗传验证.在过去国内的大多数研究中,往往以同工酶 Protoplast fusion is a technology system consisting of selection of parents, preparation of protoplasts, fusion, regeneration, establishment of marker traits, selection and verification of fusion subfamilies, etc. In this system, the genetic verification of the fusion product is a The most important step, even using auxotrophic or drug-resistant mutants as markers, provides a reliable basis for the selection of the fusion and does not represent the genetic verification of the fusion.In most of the past studies in the country , Often with isozymes