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目的探讨高脂膳食对小鼠脑内铁稳态的影响,为通过合理膳食预防和延缓脑老化提供科学依据。方法美国癌症研究所(ICR)雄性小鼠40只随机分为4组,通过Morris水迷宫实验检测各组小鼠的空间学习记忆能力,用逆转录聚合酶链反应法(RT-PCR)检测小鼠脑内铁调素(Hepcidin,Hep)mRNA的表达水平。结果高脂膳食组、脑老化模型组、高脂膳食+脑老化模型组的Hep基因与内参基因(β-actin)电泳图灰度比值分别为(0.71±0.06),(0.75±0.03)和(0.73±0.08),均低于普通膳食对照组的(0.94±0.06),差异有统计学意义(P<0.05);而高脂膳食组、脑老化模型组和高脂膳食+脑老化模型组之间Hep mRNA表达水平无明显差异。结论高脂膳食可以下调小鼠脑内Hep mRNA的表达,与脑老化模型组类似,提示高脂膳食可能引起大脑铁稳态失衡进而导致神经退行性病变。
Objective To investigate the effect of high-fat diet on iron homeostasis in mice brain and provide scientific basis for preventing and delaying brain aging through reasonable diet. Methods Forty male mice of the American Cancer Institute (ICR) were randomly divided into 4 groups. Morris water maze test was used to test the spatial learning and memory ability of each group of mice. RT-PCR was used to detect small mice. The level of hepcidin (Hep) mRNA expression in rat brain. Results The gray ratios of Hep and β-actin electrophoretograms in the high-fat diet group, brain aging model group, and high-fat diet + brain aging model group were (0.71±0.06), (0.75±0.03), and ( 0.73 ± 0.08), lower than the normal diet control group (0.94 ± 0.06), the difference was statistically significant (P <0.05); and high-fat diet group, brain aging model group and high fat diet + brain aging model group There was no significant difference in the level of Hep mRNA expression. Conclusion High-fat diet can down-regulate the expression of Hep mRNA in the brain of mice, which is similar to the brain aging model group. It suggests that high-fat diet may cause imbalance of brain iron homeostasis and further neurodegenerative diseases.