作者将聚合酶链反应(PCR)用于检测高免疫人群中可疑百日咳病人样本,并与培养、血清学试验和临床症状作了比较,评价PCR的敏感性与特异性。鼻咽分泌物用棉拭子采集,一个用于细菌培养,另一个用于PCR检测。培养按常规进行。百日咳鲍特菌PCR:引物选自于IS481 The authors used polymerase chain reaction (PCR) to detect suspicious pertussis patients in highly immunized populations and compared them with culture, serological tests and clinical symptoms to evaluate the sensitivity and specificity of PCR. Nasopharyngeal secretions were collected with cotton swabs, one for bacterial culture and the other for PCR assays. Culture routine. Bordetella pertussis PCR: Primers were selected from IS481