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探讨力竭性运动引起的氧化应激对肠功能的影响及运动性肠功能紊乱的原因,32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组EX;运动后30 min组(EX30);运动后60 min组(EX60),测定力竭性游泳后不同时相,肠组织匀浆MDA、游离巯基(Free-SH)和ATP含量。结果显示,运动后肠组织MDA含量在运动后30 min,60 min显著性增加(P<0.01);运动后Free-SH含量在运动后30min(P<0.05)和运动后60 min后(P<0.01)显著下降;运动后30 min组ATP含量显著下降(P<0.01)。结果提示,运动源性自由基产生增加,使得肠组织中游离巯基被氧化,导致ATP含量下降,可能是造成运动性肠功能紊乱的重要因素之一。
32 SD male rats were randomly divided into 4 groups: the control group (C); immediately after exercise EX group; exercise to promote oxidative stress on intestinal function and the reason of exercise-induced intestinal dysfunction, (EX30) at 30min after exercise, and EX60 at 60min after exercise. The levels of MDA, Free-SH and ATP in intestinal homogenate were measured at different phases after exhaustive swimming. The results showed that the content of MDA in the gut after exercise increased significantly at 30 min and 60 min after exercise (P <0.01), and the Free-SH content after exercise was 30 min (P <0.05) and 60 min after exercise (P < 0.01), while the content of ATP in 30 min after exercise decreased significantly (P <0.01). The results suggest that the increase of motor-derived free radicals causes the free sulfhydryl to be oxidized in intestinal tissue, resulting in the decrease of ATP content, which may be one of the important factors that cause the movement of intestinal disorders.