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实验选用SD大鼠40只,其中30只分为实验组和对照组各15只,切片用甲基绿-派洛宁染色;10只分为实验组和对照组各5只,切片用神经丝免疫组化染色.损伤大鼠大脑顶叶皮质,术时立即放入分别浸有bFGF(实验组)或PBS(0.2M pH7.2)(对照组)的海绵胶粒(1mm~3),并于术后14天,24天,34天在损伤腔内重复注射相应的实验用液25μl,术后40天取材,观察并计数神经元的数目,大脑皮质损伤腔颞侧边缘Ⅱ～Ⅲ层,距离边缘500μm范围内的脑组织,每只大鼠合计约1mm~2面积中的神经元数目,以胞体直径≥15μm具有神经元特征的,核大、园形、染色淡、位于中央、或核仁明显和胞质RNA丰富等列入计数.发现实验组神经元数目较多,对照组神经元较少,计数结果为(均数±标准差):实验组919.45±165.40/mm~2.对照组628.39±57.50/mm~2.经两样本均数t检验,P<0.05.变异系数:实验组CV
Forty Sprague-Dawley rats were selected, of which 30 were divided into experimental group and control group, 15 rats in each group. The sections were stained with methyl green-palonine. The 10 rats were divided into experimental group and control group, Immunohistochemical staining of the parietal cortex of the rat brain was performed immediately after operation, and sponge micelles (1mm ~ 3) were immersed in bFGF (experimental group) or PBS (0.2M pH7.2) (control group) At the 14th day, 24th day and 34th day after operation, 25μl of the corresponding experimental solution was repeatedly injected into the injured cavity, and the number of the neurons was counted and counted after 40 days. The temporal and temporal side Ⅱ ~ Ⅲ of cerebral cortex injury were observed, The number of neurons in a total area of about 1 mm to 2 in each brain region ranging from 500 μm in the edge to the number of neurons in a cell body with a diameter of 15 μm or more is large, The number of neurons in the experimental group was more than that in the control group, and the count results were (mean ± standard deviation): 919.45 ± 165.40 / mm ~ 2 in the experimental group Group 628.39 ± 57.50 / mm ~ 2 by two sample mean t test, P <0.05 Coefficient of variation: experimental group CV