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细胞内Ｆｏｓ蛋白表达增加，一般表明细胞活动的加强，因而Ｆｏｓ蛋白表达的分布广泛用于确定某种刺激所引起的活动加强的细胞群的所在部位。本工作采用吸入高浓度ＣＯ２后观察延髓Ｆｏｓ蛋白表达的变化的方法，初步观察ＣＯ２兴奋呼吸过程中延髓哪些核团参与活动。实验把１２只大鼠分两组进行，实验组６只吸入１４％～１５％ＣＯ２１ｈ，对照组６只吸入空气１ｈ，然后处死动物用ＡＢＣ法测定其延髓脑组织中Ｆｏｓ蛋白表达的密度。结果发现在实验组动物的延髓较广泛的区域均可见到Ｆｏｓ蛋白样免疫阳性反应（ＦＬＩ）细胞，其密度明显地超过对照组动物。ＦＬＩ细胞的分布相对集中于延髓的孤束核（ＮＴＳ）、面神经后核（ＮＲＦ）、外周橄榄腹外侧核（ＬＶＰＯ）、斜方体后核（ＮＲＴ）、巨细胞旁外侧核（ＬＰＧｉ）、外侧网状核（ＬＲｔ）、前庭内侧核（ＭＶｅ）、下橄榄内侧核Ｂ亚核（ＩＯＢ）等核团。其中尤以ＮＴＳ和ＮＲＦ区最为密集。初步提示这些核团活动的加强可能与吸入ＣＯ２的呼吸兴奋反应有关。
The increased expression of Fos protein in cells generally indicates an increase in cellular activity. Therefore, the distribution of Fos protein expression is widely used to determine the location of a population of cells that is stimulated by a stimulus. In this study, we observed the changes of Fos protein expression in medulla oblongata by inhalation of high concentration of CO2, and initially observed which nucleus of medulla oblongata involved in CO2 excitatory breathing. Twelve rats were divided into two groups. Six rats in the experimental group inhaled 14% -15% CO21h and six rats in the control group inhaled air for 1 hour. The animals were sacrificed and the density of Fos protein in the medulla oblongata was measured by ABC method. The results showed that Fos-like immunoreactive (FLI) cells were observed in the extensive medulla oblongata of experimental animals, and their density was significantly higher than that of the control animals. The distribution of FLI cells is relatively concentrated in the nucleus tractus solitarius nucleus (NTS), the posterior facial nerve nucleus (NRF), the periventricular olfactory bulbs (LVPO), the rhizome retrograde nucleus (NRT), the extracellular lateral nucleus (LPGi) Lateral reticular nucleus (LRt), medial vestibular nucleus (MVe), inferior olives medial nucleus B subunit (IOB) and other nuclei. Among them, the NTS and NRF areas are the most intensive. It is suggested that the enhancement of the activity of these nuclei may be related to the respiratory reaction of CO2 inhalation.