论文部分内容阅读
目的:研究大鼠胫骨骨折模型评价千金骨康散对骨折的治疗作用。方法:雄性SD大鼠40只,除正常组10只外,其余30只大鼠采用2%戊巴比妥钠腹腔麻醉,无菌操作于左下肢胫骨粗隆下约1 cm处,以骨剪横向将胫骨剪成3 mm宽,2 mm深的骨缺损,建立胫骨骨折模型,将造模大鼠随机分为3组,分别为模型组,千金骨康散低、高剂量组(0.35,0.7 g·kg-1),各组分别ig给药,给药体积为15 m L·kg-1,每天1次,连续给药21 d。实验末次给药后对大鼠左侧胫骨X射线成像,采血进行血液流变检测、取胫骨骨痂部位进行组织病理学检查。结果:与正常组比较,模型组大鼠有内外轻度骨痂的改变,有错位现象,大鼠血液黏度明显升高,大鼠骨折后骨痂部位组织病理学变化较为明显;与模型组比较,X成像显示千金骨康散低、高剂量能明显促进骨折愈合(P<0.05,P<0.01),高剂量能明显降低大鼠胫骨骨折模型血液黏度(P<0.05);千金骨康散低、高剂量能明显改造大鼠骨折后骨痂部位组织病理学变化(P<0.05,P<0.01)。结论:千金骨康散对大鼠胫骨骨折具有一定的促愈合作用。
Objective: To evaluate the therapeutic effect of Qianjin Gu Kang on fracture in rat model of tibia fracture. Methods: Forty male Sprague-Dawley rats were randomly divided into normal group and control group. The remaining 30 rats were anesthetized by 2% pentobarbital sodium intraperitoneally and aseptically operated at about 1 cm below the tibial tuberosity of the left lower extremity. The tibia was transversely cut into 3 mm wide and 2 mm deep bone defects. The tibial fracture model was established. The model rats were randomly divided into three groups: model group, Qianjinjikangzao low dose group (0.35,0.7 g · kg-1). The rats in each group were administered ig with the volume of 15 m L · kg-1 once a day for 21 days. After the last administration of the experiment, the left tibia of the rat was imaged by X-ray and blood was collected for blood rheological examination. The tibia callus was examined by histopathology. Results: Compared with the normal group, the rats in the model group had slight callus changes inside and outside, with dislocation phenomenon, and the blood viscosity of the rats increased obviously. The histopathological changes in the callus area of the rats after fracture were more obvious. Compared with the model group (P <0.05, P <0.01). The high dose could significantly reduce the blood viscosity of the tibia fracture model in rats (P <0.05), and the low dose of Qianjin Gu Kang , High dose can obviously remodeling histopathological changes of callus after fracture in rats (P <0.05, P <0.01). Conclusion: Qianjingu Kang San has a certain healing effect on tibial fractures in rats.