目的 :建立简单、有效的猪血小板衍生生长因子 (p PDGF)纯化方法。方法 :采用超声裂解、酸醇抽提、离子交换色谱、凝胶色谱等方法提纯 p PDGF,用 SDS-聚丙烯酰胺凝胶电泳测定其相对分子质量 ,并检测其生物学活性。结果 :纯化的 p PDGF相对分子质量为 (2 .879～ 3.0 78)× 10 4,纯化倍数为 75 82倍 ,活性回收率为 3% ,比活性为 3.41176× 10 5 U/ mg。 结论 :提取和纯化 p PDGF的方法简单 ,且达到较好的结果 ,为进一步研究 PDGF的理化性质及生物学特性打下基础。 Objective: To establish a simple and effective purification method of porcine platelet-derived growth factor (p PDGF). Methods: The p PDGF was purified by sonication, acid-alcohol extraction, ion-exchange chromatography and gel chromatography. The relative molecular mass was determined by SDS-polyacrylamide gel electrophoresis and its biological activity was tested. Results: The relative molecular mass of purified p PDGF was (2.889 ~ 3.078) × 10 4, the purification fold was 75 82-fold, the activity recovery was 3% and the specific activity was 3.41176 × 10 5 U / mg. Conclusion: The method of extracting and purifying p PDGF is simple and achieves good results, which lays the foundation for further study on the physicochemical properties and biological characteristics of PDGF.