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本文以1/2Hoagland营养液栽培的小金海棠为试材,研究70 mmol·L-1的NaCl胁迫下,钙对小金海棠幼苗生物量、超氧自由基(O2.-)产生速率、丙二醛(MDA)含量、电解质相对渗透率、抗氧化酶(SOD、POD、CAT和APX)活性及可溶性蛋白含量的影响。结果表明,盐胁迫下,小金海棠幼苗生物量显著低于对照,根系和叶片的O2.-产生速率、MDA含量、电解质相对渗透率、抗氧化酶活性及可溶性蛋白含量显著高于对照。盐胁迫下,与不加钙处理相比,加钙处理显著降低了小金海棠幼苗O2.-产生速率、MDA含量及电解质相对渗透率,显著提高了生物量、抗氧化酶和可溶性蛋白的含量,10 mmol·L-1 CaCl2处理的效果显著好于30 mmol·L-1处理的。综上可知,盐胁迫下小金海棠幼苗的生长受到抑制,外源施钙可以减轻盐胁迫对幼苗造成的伤害,提高幼苗对盐胁迫的适应能力。
In this paper, the culture medium of Haliotisides Hortensis with 1 / 2Hoagland nutrient solution was used to study the effects of calcium on the biomass, the production rate of superoxide radical (O2.-), the content of malondialdehyde (MDA), electrolyte relative permeability, antioxidant enzymes (SOD, POD, CAT and APX) activity and soluble protein content. The results showed that under salt stress, the biomass of seedlings of Begoniaceae was significantly lower than that of the control. The O2.- production rate, MDA content, electrolyte relative permeability, antioxidant enzyme activities and soluble protein contents of roots and leaves were significantly higher than those of the control. Under salt stress, compared with no calcium treatment, Ca2 + treatment significantly reduced O2.- production rate, MDA content and electrolyte relative permeability, and significantly increased biomass, antioxidant enzymes and soluble protein content, The effect of 10 mmol·L-1 CaCl2 treatment was significantly better than that of 30 mmol·L-1 treatment. In conclusion, under the salt stress, the growth of seedlings was inhibited, and exogenous calcium could reduce the damage caused by salt stress on the seedlings and improve the ability of seedlings to adapt to salt stress.