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目的 :探讨不同来源、不同致病性的皮炎外瓶霉在基因学上是否具有差异性。方法 :试验菌株包括皮炎外瓶霉 1 6株 (模式株、标准株、临床及自然分离株 ) ,甄氏外瓶霉 1株 (标准株 ) ,丛梗孢外瓶霉 1株 (临床株 )。煮沸冷冻法提取DNA ,常规PCR扩增核糖体基因及其转录间隔区 ,应用限制性片段长度多态性、DNA多序列分析方法等进行研究。结果 :皮炎外瓶霉核糖体基因及其转录间隔区较为保守 ,PCR RFLP对于该菌种的鉴定具有较大意义 ;DNA序列分析显示 ,不同来源、不同致病性的皮炎外瓶霉分属于不同的生物群落 ,提示不同群落致病性的差异存在一定的遗传学基础。结论 :皮炎外瓶霉核糖体基因及其转录间隔区序列保守 ,对于该菌种的分类鉴别具有重要的意义
Objective: To investigate whether there are differences in genetics between different sources and pathogenic dermatitis. Methods: The test strains included 16 strains of Streptomyces epidermidis (model strain, standard strain, clinical and natural isolates), 1 strain of Echinococcus oblonga (standard strain), 1 strain of Streptomyces polymyxa (clinical strain) . The DNA was extracted by boiling and freezing method. The ribosomal gene and its transcribed spacer were amplified by conventional PCR. The restriction fragment length polymorphism and DNA multi - sequence analysis were used to study. Results: The ribosomal DNA gene and its transcribed spacer region were more conserved in Streptococcus pneumoniae. PCR RFLP was of great significance for the identification of the strain. DNA sequence analysis showed that different sources and different pathogenic strains Of the biome, suggesting that there is a certain genetic basis for the differences in pathogenicity of different communities. CONCLUSION: The conserved sequence of ribosomal genes and their transcribed spacer regions in Streptococcus pneumoniae is of great significance for the classification and identification of this strain