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目的:研究兴安落叶松中阿拉伯半乳聚糖硫酸酯(AGS)对S_(180)小鼠免疫功能的影响。方法:以黄芪多糖为阳性对照药,采用红细胞免疫粘附法检测AGS对荷瘤小鼠红细胞免疫调节因子的影响;以YAC-1为靶细胞检测AGS对NK细胞杀伤肿瘤细胞的功能的影响;ELISA法检测荷瘤小鼠血清中细胞因子TNF-α和IFN-γ的变化,比较AGS对细胞因子含量的影响。结果:各AGS给药组中促进率和抑制率有不同程度的改变,其中以AGS高剂量(400mg/kg·d)对RBGC3b受体的抑制率改变最为明显(39.59±7.33);AGS明显增强荷瘤小鼠NK细胞的杀伤能力,AGS低剂量组(100mg/kg·d)效果最好(44.72±10.98);AGS能够明显诱导荷瘤小鼠体内细胞因子TNF-α的和IFN-γ的生成,AGS高剂量(400mg/kg·d)组效果最好,TNF-α的和IFN-γ生成量分别为30.33±6.59和103.5±18.41)。各剂量组均差异明显,具有统计学意义。结论:AGS的抗肿瘤作用与其增强荷瘤机体的免疫功能有关。
Objective: To study the effect of middle-aged arabinogalactan sulfate (AGS) on immune function in S 180 mice. Methods: Astragalus polysaccharide was used as a positive control drug. The effect of AGS on erythrocyte immune regulatory factor in tumor-bearing mice was detected by erythrocyte immunoadsorption assay. The effect of AGS on cytotoxicity of NK cells on tumor cells was detected by YAC-1 as target cell. The serum levels of cytokines TNF-α and IFN-γ in tumor-bearing mice were detected by ELISA, and the effect of AGS on cytokines was compared. Results: The rate of AGS administration and the rate of inhibition varied to different degrees. Among them, AGS high dose (400mg / kg · d) had the most obvious inhibitory effect on RBGC3b receptor (39.59 ± 7.33), AGS significantly increased The cytotoxicity of NK cells in tumor-bearing mice was the best (44.72 ± 10.98) for AGS low dose group (100mg / kg · d). AGS could obviously induce TNF-α and IFN-γ AGS high dose (400mg / kg · d) group produced the best effect, the production of TNF-α and IFN-γ were 30.33 ± 6.59 and 103.5 ± 18.41, respectively). Each dose group were significantly different, with statistical significance. Conclusion: The anti-tumor effect of AGS is related to the enhancement of immune function of tumor-bearing body.