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目的研究羟基磷灰石纳米粒子(nano-HAP)对人肝癌BEL-7402细胞和结肠癌SW- 480细胞生长及凋亡的影响。方法用羟基磷灰石纳米粒子以不同终浓度作用于这二株细胞。用噻唑蓝(MTT)比色法观察其对两株细胞的生长抑制,荧光显微镜、透射电镜、DNA琼脂糖凝胶电泳、流式细胞术(FCM)等方法从细胞凋亡的角度探讨其作用机制。结果羟基磷灰石纳米粒子对 BEL-7402和SW-480细胞的半数有效抑制浓度(IC_(50))值分别为29．28 mg／L和36．66 mg/L。50～ 100 mg／L的纳米粒子处理48 h后。癌细胞即可表现出细胞皱缩、核质浓缩、核碎裂以及凋亡小体形成等凋亡特征性形态改变。作用48 h后,琼脂糖凝胶电泳观察到DNA“梯带”。流式细胞仪定量分析显示人肝癌、结肠癌细胞经0、50、100、200 mg／L作用48 h后的细胞凋亡率分别为2．23％、 20．35％、29．34％、53．64％和3．57％、21．45％、37．10％、49．45％。结论羟基磷灰石纳米粒子对人肝癌BEL-7402和结肠癌SW-480细胞有明显的生长抑制作用,诱导癌细胞凋亡可能是其主要作用机制。
Objective To study the effect of nano-HAP on the growth and apoptosis of human hepatocellular carcinoma BEL-7402 cells and colon cancer SW-480 cells. Methods Hydroxyapatite nanoparticles were applied to these two cells at different final concentrations. The growth inhibition of the two cell lines was observed by MTT colorimetric assay. The effects of apoptosis on the two cell lines were observed by fluorescence microscopy, transmission electron microscopy, DNA agarose gel electrophoresis and flow cytometry (FCM) mechanism. Results The half inhibitory concentration (IC 50) of hydroxyapatite nanoparticles on BEL-7402 and SW-480 cells were 29.28 mg / L and 36.66 mg / L, respectively. 50 ~ 100 mg / L nanoparticles after 48 h treatment. Cancer cells can show cell shrinkage, condensed nuclei, nuclear fragmentation and apoptotic body formation and other apoptotic morphological changes. After 48 h, DNA “ladder” was observed by agarose gel electrophoresis. Flow cytometry quantitative analysis showed that the apoptotic rates of human hepatoma and colon cancer cells after treated with 0, 50, 100, 200 mg / L for 48 h were 2.23%, 20.35%, 29.34% 53.64% and 3.57%, 21.45%, 37.10% and 49.45% respectively. Conclusion Hydroxyapatite nanoparticles can significantly inhibit the growth of human hepatocellular carcinoma BEL-7402 and colon cancer SW-480 cells. Inducing apoptosis of cancer cells may be the main mechanism.