抗牛病毒性腹泻病毒单克隆抗体的制备及双抗夹心ELISA检测方法的建立

来源 :中国预防兽医学报 | 被引量 : 0次 | 上传用户：highbird51426

【摘要】

：

为建立牛病毒性腹泻病毒(BVDV)双抗夹心ELISA检测方法,本研究将BVDV 890病毒株(BVDV-2)浓缩并纯化后免疫BALB/c小鼠,经常规技术进行细胞融合并筛选得到一株稳定分泌IgG的杂交

【作者】

：

蒋颖林燕清陶洁孟祥升段小丽王娟王银张信军王建

【机构】

：

扬州大学兽医学院,江苏扬州225009中国兽医药品监察所,北京100081;扬州大学兽医学院,江苏扬州225009;浙江诺倍威生物技术有限公司,浙江杭州310018;扬州大学兽医学院,江苏扬州,225

【出处】

：

中国预防兽医学报

【发表日期】

：

2012年12期

【关键词】

：

牛病毒性腹泻病毒 E2蛋白单克隆抗体双抗体夹心ELISA

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论文部分内容阅读

为建立牛病毒性腹泻病毒(BVDV)双抗夹心ELISA检测方法,本研究将BVDV 890病毒株(BVDV-2)浓缩并纯化后免疫BALB/c小鼠,经常规技术进行细胞融合并筛选得到一株稳定分泌IgG的杂交瘤细胞株,命名为3F9.以BVDV单克隆抗体(MAb) IgM为捕获抗体,生物素标记的3F9为检测抗体,初步建立BVDV特异的双抗体夹心ELISA检测方法(BAS-ELISA).采用建立的BAS-ELISA方法检测35份临床病牛血清样品,检出阳性样本13份;与RT-PCR的符合率达到94.29％;表明本研究所建立的BAS-ELISA方法可用于BVDV感染的临床诊断,为BVDV的免疫学研究奠定了基础.

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