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目的 观察普鲁卡因和利多卡因对细胞内织网内ryanodine敏感的钙离子释放的影响。方法 采用 60 -80g的蒙古鼠海马切片 ,用显微荧光法测定细胞内钙离子浓度。将切片用含 50mmol/LKCl的人工脑脊液灌注 3 0秒 ,然后用人工脑脊液灌注 ,5分钟后用含 2 0mmol/L咖啡因的脑脊液灌注 2分钟 ,然后换为人工脑脊液直到实验结束。将 1 0 0umol/L的普鲁卡因或利多卡因分别加入到高钾后的灌注液中观察它们对钙离子释放的影响。结果 咖啡因可致细胞内钙离子浓度的明显增高 ,而普鲁卡因可使这种增高减少 1 2 % ,而利多卡因则无此抑制作用。结论 普鲁卡因可抑制ryanodine受体介导的细胞内钙离子释放 ,而利多卡因则可能通过其他机理抑制细胞内钙离子的释放
Objective To investigate the effects of procaine and lidocaine on ryanodine-sensitive calcium release in intracellular meshes. Methods 60-80g Mongolian rat hippocampal slices were used to measure intracellular calcium concentration by micro-fluorescence method. The sections were perfused with artificial cerebrospinal fluid containing 50 mmol / L KCl for 30 seconds and then perfused with artificial cerebrospinal fluid. After 5 minutes, the cerebrospinal fluid containing 20 mmol / L caffeine was infused for 2 minutes and then replaced with artificial cerebrospinal fluid until the experiment was completed. The effects of calcium ion release were observed by adding 100 umol / L of procaine or lidocaine to perfusate after high potassium, respectively. Results Caffeine induced a significant increase in intracellular calcium concentration, whereas procaine reduced this increase by 12%, whereas lidocaine did not. Conclusion Procaine inhibits ryanodine receptor-mediated intracellular calcium release, whereas lidocaine may inhibit intracellular calcium release by other mechanisms