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背景:辣椒素外敷治疗浅表性疼痛已为人们接受,但能否直接作用于皮下或深层组织中的神经末梢或神经治疗三叉神经痛?目的:观察皮下注射辣椒素对大鼠面部皮肤降钙素基因相关肽、一氧化氮合酶阳性神经纤维的影响。设计:随机对照实验。单位:咸宁学院医学院解剖学教研室,华中科技大学同济医学院神经生物学研究室。材料:实验于2003-10/12于华中科技大学同济医学院神经生物学研究室完成。健康Wistar大鼠20只,雌雄不拘,体质量120～170g。方法:辣椒素皮下注射处理大鼠三叉神经眶下支,大鼠左侧面区为对照侧,右侧面区为实验侧,按用量将动物分为辣椒素20μL组,辣椒素30μL组,辣椒素50μL组,辣椒素100μL组4组,每组5只。24h取材后切片镜检,降钙素基因相关肽和一氧化氮合酶免疫组化染色。主要观察指标:①镜检实验侧各组染色标本中降钙素基因相关肽和一氧化氮合酶阳性神经纤维的变化;图像分析降钙素基因相关肽和一氧化氮合酶的平均吸光度。②大鼠的特征性行为和体征改变。结果:20只大鼠均进入结果分析。①行为变化:皮下注射辣椒素溶液数分钟后,大鼠出现一系列特征性的行为和体征改变,但随着用药次数的增加,则逐渐减轻或消失。②镜检结果:实验侧各组标本中降钙素基因相关肽和一氧化氮合酶阳性神经纤维有明显差别。③图像分析各组大鼠面部皮肤降钙素基因相关肽平均吸光度:对照侧为0.984±0.056,辣椒素20μL组为0.947±0.025,辣椒素30μL组为0.852±0.042,辣椒素50μL组为0.756±0.028,辣椒素100μL组为0.730±0.016;各组大鼠面部皮肤一氧化氮合酶平均吸光度:对照侧为0.151±0.009,辣椒素20μL组为0.148±0.007,辣椒素30μL组为0.132±0.012,辣椒素50μL组为0.111±0.067,辣椒素100μL组为0.107±0.006。方差分析组间差异有显著性意义(P<0.01)。结论:降钙素基因相关肽、一氧化氮合酶参与了伤害性信息处理和调控痛与镇痛的过程,辣椒素通过耗竭大量的神经递质而发挥镇痛作用。
BACKGROUND: Capsaicin topical treatment of superficial pain has been accepted, but can directly affect the subcutaneous or deep tissue of the nerve endings or nerve treatment of trigeminal neuralgia? Objective: To observe the subcutaneous injection of capsaicin on rat facial skin calcification Superoxide dismutase gene, Nitric oxide synthase positive nerve fiber Design: Randomized controlled experiment. SETTING: Department of Anatomy, Xianning University Medical College, Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology. MATERIALS: The experiment was performed at the Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology from October to December 2003. Healthy Wistar rats 20, male or female, body weight 120 ~ 170g. Methods: Subcutaneous injection of capsaicin into the infraorbital branch of the trigeminal nerve in rats. The left side of the rats was the control side and the right side of the side was the experimental side. The animals were divided into capsaicin 20 μL group, capsaicin 30 μL group, 50μL group, capsaicin 100μL group 4, each group 5. Histological examination was made after 24h, calcitonin gene-related peptide and nitric oxide synthase immunohistochemical staining. MAIN OUTCOME MEASURES: (1) The changes of calcitonin gene-related peptide and nitric oxide synthase positive nerve fibers in each group on microscopic examination; the average absorbance of calcitonin gene-related peptide and nitric oxide synthase were analyzed by image analysis. ② rat characteristic behavior and signs change. Results: Twenty rats entered the result analysis. ① behavior changes: Subcutaneous injection of capsaicin solution for a few minutes, the rats showed a series of characteristic behavior and signs of change, but with the increase in the number of medication, then gradually reduce or disappear. ② microscopic examination results: experimental side of each group of specimens of calcitonin gene-related peptide and nitric oxide synthase positive nerve fibers were significantly different. ③ Image analysis The average absorbance of calcitonin gene-related peptide in facial skin of rats in each group was 0.984 ± 0.056 on the control side, 0.947 ± 0.025 in 20μL capsaicin group, 0.852 ± 0.042 in 30μL capsaicin group and 0.756 ± 50μg in capsaicin 50μL group 0.028, and capsaicin 100μL group was 0.730 ± 0.016. The mean absorbance of facial nitric oxide synthase in each group was 0.151 ± 0.009 on the control side, 0.148 ± 0.007 in 20μL capsaicin group, 0.132 ± 0.012 in 30μL capsaicin group, The capsaicin 50 μL group was 0.111 ± 0.067, and the capsaicin 100 μL group was 0.107 ± 0.006. Analysis of variance showed significant differences between groups (P <0.01). CONCLUSION: Calcitonin gene-related peptide and nitric oxide synthase are involved in the process of nociceptive information and regulation of pain and analgesia. Capsaicin exerts analgesic effect by depleting large amounts of neurotransmitter.