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用中国春单体系列和二体中国春作为母本与Orofen杂交。选择出所有类型的单体杂种F_1植株,令其自交结实。在温室内(10—25℃)用秆锈菌小种21C3和34C2的单孢菌系分别接种鉴定各杂交组合的F_2代苗期的抗性分离表现。对小种21C3,除2D和6D之外,其它单体类型和二体对照的F_2代都符合抗病15:1感病的分离比例;对小种34C2,除2D之外,其它单体类型和二体对照的F_2代都符合抗病3:1感病的分离比例。用Orofen与含有国际上已定位于2D和6D染色体上的已知Sr基因的品系(或品种)杂交。对小种21C3,Orofen与含有Sr5和Sr6的单基因系的杂交F_2未分离出感病的植株;对小种34C2,只有与含有Sr6的单基因系的杂交F_2代未分离出感病的植株。这表明,Orofen在2D染色体上含有Sr6,它兼抗小种21C3和34C2,分别提供0—1;1~(++)x~-和;1—;1~(++)x~-的抗性效应;而在6D染色体上携带抗病基因Sr5,它只抗小种21C3.控制0—;1~-的侵染型。对无毒性的小种,Sr5对Sr6的抗病效应是上位的。
Using Chinese Spring Monomer Series and Chinese Spring as the female parent to cross with Orofen. Select all types of monomeric hybrid F_1 plants to self-fertility. The resistance segregation of F_2 generation at seedling stage of each cross was identified by inoculation with Monocultures of Stem rust races 21C3 and 34C2 in greenhouse (10-25 ℃). For the race 21C3, F2 and 6D other than D2 and 6D all met the segregation ratio of resistance-resistant 15: 1 susceptible lines. For race 34C2, except for 2D, other monomer types F_2 generation with two-body control were all in line with the segregation ratio of 3: 1 susceptible disease. Hybrids were made with Orofen with lines (or varieties) containing known Sr genes internationally located on both the 2D and 6D chromosomes. The susceptible plants were not isolated from hybrid F_2 of races 21C3, Orofen and single gene lines containing Sr5 and Sr6. The susceptible plants were not isolated from hybrid F_2 of races 34C2 and only single lines with Sr6 . This indicates that Orofen contains Sr6 on the 2D chromosome, which is resistant to races 21C3 and 34C2 and provides 0-1; (++) x- and 1-; Resistant effect; and on the 6D chromosome carrying disease-resistant gene Sr5, it only resistant race 21C3. Control 0-; 1 ~ - infection. For the non-toxic races, the resistance of Sr5 to Sr6 is superior.