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目的确定新疆葡萄树伤流液多糖含量,探究吐鲁番无核白和喀什木纳格萄萄树伤流液多糖及其纯化组分的抗氧化活性。方法采用蒽酮-硫酸法检测多糖含量,利用醇沉法和改进的Sevage法制备粗多糖,经过DEAE-52纤维素柱对粗多糖进行分离纯化,以羟自由基、超氧自由基、DPPH·自由基、亚硝基自由基、ABTS·自由基为指标检测多糖组分及原液的抗氧化能力。结果吐鲁番无核白和喀什木纳格葡萄树伤流液中多糖含量分别为114和193μg·mL-1,经过DEAE-52纤维素柱分离分别得到8个多糖组分,其中两者4个含量较高的多糖组分和原液都具有不同程度的抗氧化活性,且对羟自由基的清除能力强于对其他4种自由基的清除能力,两地葡萄树伤流液的抗氧化活性存在差异。结论吐鲁番无核白和喀什木纳格葡萄树伤流液的多糖组分均具有一定的抗氧化活性,并且其抗氧化活性存在差异。
Aim To determine the content of polysaccharide in wound fluid of Vitis vinifera L.in Xinjiang, and to study the antioxidant activity of Polysaccharides from Purpurae of Turpan and its purification components. Methods Anthrone-sulfuric acid method was used to detect the content of polysaccharides. The crude polysaccharides were prepared by ethanol precipitation method and modified Sevage method. The crude polysaccharides were separated and purified by DEAE-52 cellulose column. The hydroxyl radical, superoxide radical, DPPH · Free radical, nitroso free radical, ABTS · free radical as indicators to detect the antioxidant capacity of polysaccharide components and stock solution. Results The contents of polysaccharides in Turion seedless and Kashmir grape trees were 114 and 193μg · mL-1, respectively. Eight polysaccharides were separated by DEAE-52 cellulose column, of which four were The higher polysaccharide components and stock solutions all had different degrees of antioxidant activity, and the ability to scavenge hydroxyl radicals was stronger than that of the other four free radicals. There was a difference in the antioxidant activity of grape juice . Conclusion The polysaccharide components of Turion seedless grape and Kashi Mangag grape waste fluid all have certain antioxidant activity, and their antioxidant activity is different.