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目的分析华支睾吸虫感染小鼠肝脏中Nrf2和Trx1编码基因的表达情况,初步探讨Nrf2及Trx1在华支睾吸虫致病过程中的作用。方法选用健康Balb/c小鼠建立华支睾吸虫感染模型,分别在实验第0、3、5、7、10、14、21、28和35d摘除小鼠眼球采血,分离血清,检测血清中天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性;颈椎脱臼处死小鼠取肝脏,采用逆转录聚合酶链反应(RT-PCR)检测肝脏中Nrf2和Trx1的m RNA表达情况。结果与对照组相比,感染组小鼠在感染第5、7、10、14、21和35d血清ALT活性显著升高(P<0.05);分别为(19.9332±1.98180)U/L、(64.3699±14.53874)U/L、(46.7455±6.27572)U/L、(99.0038±25.34329)U/L、(46.7590±8.85478)U/L和(50.3374±14.19886)U/L。感染组小鼠抗氧化应激通路上游基因Nrf2和下游Trx1的m RNA表达升高;其中Nrf2在感染第5天升高显著(P<0.05);Trx1在感染第21d升高显著(P<0.05)。结论 Nrf2和Trx1 m RNA在华支睾吸虫感染过程中表达升高,提示Nrf2/Trx1通路可能在华支睾吸虫致病过程中发挥一定的作用。
Objective To analyze the expression of Nrf2 and Trx1 genes in the liver of Clonorchis sinensis infected mice and to explore the role of Nrf2 and Trx1 in pathogenicity of Clonorchis sinensis. Methods Healthy Balb / c mice were used to establish Clonorchis sinensis infection model. Blood samples were taken from the eyeballs of mice at 0, 3, 5, 7, 10, 14, 21, 28 and 35 days after experiment, serums were separated, (AST) and alanine aminotransferase (ALT) activity. The mice in the cervical dislocation were sacrificed and the liver was taken. The mRNA of Nrf2 and Trx1 in the liver was detected by reverse transcription polymerase chain reaction (RT-PCR) Express the situation. Results Compared with the control group, the ALT activity in sera of infected mice was significantly increased at the 5th, 7th, 10th, 14th, 21st and 35th days after infection (P <0.05), respectively (19.9332 ± 1.98180) U / L, (64.3699 ± 14.53874 U / L, 46.7455 ± 6.27572 U / L, 99.0038 ± 25.34329 U / L, 46.7590 ± 8.85478 U / L and 50.3374 ± 14.19886 U / L, respectively. The mRNA expression of Nrf2 and downstream Trx1 in the infected group increased significantly (P <0.05). The level of Trx1 increased significantly on the 21st day (P <0.05) ). Conclusion The expression of Nrf2 and Trx1 m RNA in Clonorchis sinensis is increased, suggesting that Nrf2 / Trx1 pathway may play a role in the process of Clonorchis sinensis.