论文部分内容阅读
以籼稻单倍体无性系做芽为材料，研究了激素及秋水仙碱等处理对籼稻单倍体微芽的诱导、分化、扩增及加倍的影响。2mg／L的2，4－D可提高籼稻单倍体微芽的培养力。NAA对税稻单倍体微芽的扩增有明显影响，0．5mg／L的NAA可成倍扩增微芽，培养35d后，其芽数比原始芽数增加了46．21倍，比不加NAA的对照增加了2．8倍，巨单芽重仅0．079mg。籼稻单倍体微芽扩增的适宜培养基为：N6附加MET2．5mg／L、NAA0.5mg／L、6－BA2mg／L。500mg／L秋水仙碱溶液处理籼稻单倍体微芽48h，其二倍体得率较高，绿苗率及二倍体率分别为42．9％和60．0％；秋水仙碱处理愈伤组织的效果不佳，虽然提高秋水仙碱处理浓度可提高二倍体率，最高可达100％，但由于绿苗分化率下降，使总的二倍体得苗率比不处理的对照低。
Indica haploid clones were used as shoot materials to study the effects of hormones and colchicine on induction, differentiation, expansion and doubling of haploid micro shoots in indica rice. 2,4-D at 2 mg / L could improve the culture ability of haploid micro-shoots in indica rice. NAA had a significant effect on the haploid micro-buds of tax rice. NAA 0.5 mg / L could multiply the micro-buds multiply. After 35 days of culture, the number of shoots increased 46.21 times than that of the original ones The control without NAA increased 2.8-fold and the giant single bud weight was only 0.079 mg. Indica rice haploid microspore suitable culture medium: N6 additional MET2.5mg / L, NAA0.5mg / L, 6-BA2mg / L. 500mg / L colchicine solution treatment of indica haploid micro-buds 48h, the diploid yield was higher, the rate of green seedlings and diploid rates were 42.9% and 60.0%; colchicine treatment of callus Tissue was not effective, although increasing the concentration of colchicine can increase the diploid rate up to 100%, but due to the decline of green plantlet differentiation rate, the total diploid seedling rate is lower than the untreated control.