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目的 :研究小肝癌中端粒酶蛋白 h TRT基因与 P5 3蛋白、N- ras蛋白、 nm2 3H1蛋白、PCNA表达及其相关性。方法 :在 2 0例小肝癌中用原位杂交 ,免疫组化方法检测 h TRT基因与 P5 3蛋白、N- ras蛋白、nm2 3H1蛋白、PCNA的表达。结果 :h TRT阳性信号检出率 80 % (16 / 2 0 ) ,其余蛋白检出阳性率 P5 3为 35 % (7/ 2 0 ) ,N- ras为90 % (18/ 2 0 ) ,nm 2 3H1为 85 % (17/ 2 0 )及 PCNA强阳性率为 5 5 % (11/ 2 0 ) ;癌中 h TRT阳性信号、P5 3蛋白、PCNA表达强度显著高于癌旁非癌组织 (P<0 .0 5 ) ;癌不同分化组中 P5 3蛋白、nm2 3H1、PCNA表达有显著差异 (P<0 .0 5 ) ;癌中 h TRT阳性信号分布有膜型和浆型两种形式 ,其分布形式、信号强度均与 PCNA阳性强度密切相关 (P<0 .0 1) ,但未检出与 P5 3、N- ras、nm 2 3H1有明显相关性。 nm2 3H1,N- ras在癌与非癌中表达较高。结论 :肝癌发生发展不同阶段有多种基因各自发挥着不同的作用 ,P5 3异常表达是肝癌多基因变化的基础。端粒酶活化发生在肝癌早期 ,是肝细胞增生和恶性转化所必需 ,其检出结果对鉴别肿瘤恶变有重要意义。
Objective : To study the expression of telomerase protein h TRT gene and P53 protein, N-ras protein, nm23H1 protein and PCNA in small hepatocellular carcinoma and their correlation. Methods: The expression of hTRT gene and P53 protein, N-ras protein, nm23H1 protein and PCNA were detected by in situ hybridization in 20 cases of small hepatocellular carcinoma. RESULTS: The detection rate of h TRT positive signal was 80% (16/20). The positive rate of other proteins detected P3 3 was 35% (7/20), N-ras was 90% (18/20), nm. 2 3H1 was 85% (17/20) and PCNA strong positive rate was 5 5% (11/20); h TRT positive signal, P53 protein, and PCNA expression intensity in cancer were significantly higher than those in adjacent noncancer tissues ( P<0.05); P53 protein, nm23H1, and PCNA expression were significantly different in different cancer differentiation groups (P<0.05); hTRT positive signal distribution in cancer had two forms: membrane type and plasma type. The distribution pattern and signal intensity were closely related to the positive intensity of PCNA (P<0.01), but no significant correlation was found with P53, N-ras, and nm23H1. Nm2 3H1, N-ras is highly expressed in cancer and non-cancer. Conclusion: There are multiple genes in different stages of HCC development. P5 3 abnormal expression is the basis of multiple gene changes in HCC. Telomerase activation occurs in early stage of liver cancer and is necessary for hepatocyte proliferation and malignant transformation. The detection results are important for the identification of malignant tumors.