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目的观察细菌脂多糖(lipopolysaccharide,LPS)、脂蛋白(bacteriallipoprotein,BLP)及其DNA〔鸟嘌呤二核苷酸(CpG)寡核苷酸(oligonucleoetide),CpG-ODN〕的体内协同致病作用。方法147只小鼠随机分为等渗盐水对照组、单纯LPS组、单纯CpG-ODN组、单纯BLP组、LPS+BLP组、LPS+CpG-ODN组和LPS+BLP+CpG-ODN组,尾静脉注射给药,观察小鼠死亡率及血清肿瘤坏死因子(TNF)-α水平。结果注射后12,24,48h,二联组的死亡率显著高于单独给药组(P<0.05或P<0.01);三联给药组各时相点死亡率显著高于二联组(P<0.05)。单独给药后6h,TNF-α水平均显著高于对照组(P<0.05),12,24h与对照组比较,差异无统计学意义。二联组与单因素组和对照组比较,6,12h血TNF-α均明显升高,差异有统计学意义(P<0.05)。三者联合刺激时,6,12h血TNF-α与二联组比较,差异有统计学意义(P<0.05),至24h仍显著高于对照组(P<0.05)。结论细菌LPS、细菌脂蛋白和细菌DNA不仅自身存在一定的致病能力,而且三者间具有明显的协同效应,特别是三者联合给药时作用最强。
Objective To observe the synergistic pathogenicity of bacterial lipopolysaccharide (LPS), bacterial lipoprotein (BLP) and DNA (CpG ODN). Methods 147 mice were randomly divided into normal saline control group, LPS group, CpG-ODN group, BLP group, LPS + BLP group, LPS + CpG-ODN group and LPS + BLP + Intravenous injection, observed the mortality of mice and serum tumor necrosis factor (TNF) -α levels. Results The mortality rates in the combined group were significantly higher than those in the untreated group (P <0.05 or P <0.01) at 12, 24 and 48 h after injection. The mortality rates in the triple combined group at each time point were significantly higher than those in the combined group <0.05). At 6h after administration, the level of TNF-α was significantly higher than that of the control group (P <0.05). There was no significant difference between the two groups at 12 and 24 hours. Compared with univariate group and control group, the levels of TNF-α at 6 and 12 h were significantly increased in the combined group (P <0.05). The levels of TNF-α at 6 and 12h were significantly different from those in the combined group (P <0.05), and were still significantly higher at 24h and 12h than those in the control group (P <0.05). Conclusion Bacterial LPS, bacterial lipoprotein and bacterial DNA not only have certain pathogenicity, but also have obvious synergistic effect among the three, especially the combination of the three is the strongest.