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利用体外培养细胞转化试验,测试致癌因子和研究癌变机理,已经成为毒理学和肿瘤研究的一个新领域。虽然在四十年前,Earle发现3-甲基胆蒽能促使C3H小鼠细胞和其它细胞株恶性转化,但这种小鼠细胞自发转化率很高,因此认为用培养细胞作化学致癌作用的研究是不可靠的,就此中断了20年。1963年Berwald和Sachs报道用叙利亚金黄地鼠胚胎(SHE)细胞作
It has become a new field of toxicology and tumor research that using in vitro cultured cell transformation test, testing carcinogenic factor and studying carcinogenesis mechanism. Although earle found that 3-methylcholanthrene promoted the malignant transformation of C3H mouse and other cell lines 40 years ago, Earle’s mouse spontaneous conversion rate is so high that it is believed that the use of cultured cells for chemical carcinogenesis The study is not reliable, so interrupted for 20 years. 1963 Berwald and Sachs report the use of Syrian hamster embryo (SHE) cells