以旋毛虫肌肉期幼虫的分泌排泄抗原（ES）和纯化ES中46～58KD抗原（P－ES）为诊断抗原，用Dot－ELISA同时检测34份旋毛虫病人血清，阳性检出率分别为91．18％和94．12％（P＞0．05）．而100份正常人血清均为阴性；检测其它4种蠕虫病人血清62份，交叉反应率分别为29．3％和0％，P－ES的特异性明显优于ES（P＜0．05）。另外，以P-ES为珍断抗原，比较Dot-ELISA和间接ELISA用于诊断旋毛虫病人的检测效果，实验表明：Dot-ELISA不仅有间接ELISA相似的敏感性和特异性，而且简便、快速、经济，更适合于推广应用。 Serum samples from 34 patients with Trichinella spiralis were detected by Dot-ELISA using secreted antigen (ES) of Trichinella spiralis larvae and 46-58KD antigen (P-ES) in purified ES, respectively. The positive detection rates were 91 .18% and 94.12% (P> 0.05). While the serum levels of all the 100 normal individuals were negative. The serum levels of 62 isolates from other 4 worms were 29.3% and 0%, respectively. The specificity of P-ES was significantly better than that of ES (P <0.05) . In addition, using P-ES as the rare-earth antigen and Dot-ELISA and indirect ELISA for the diagnosis of Trichinella inpatients, Dot-ELISA not only has the similar sensitivity and specificity of indirect ELISA, but also is simple and rapid , Economy, more suitable for the promotion and application.