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用分子病毒学方法对 HBV 的 DNA 序列进行分析证明,克隆的 HBV DNA 与 HBV粒子的 DNA 不同。克隆的 HBV DNA 没有缺口结构和共价连结的蛋白质,因而有人提出关于克隆的 HBV DNA 能否在体内外复制的问题。
Analysis of the DNA sequence of HBV by molecular virological methods shows that the cloned HBV DNA differs from that of HBV particles. Cloned HBV DNA has no nicked structure and covalently linked proteins, so a question has been raised as to whether cloned HBV DNA can be replicated in vitro or in vivo.