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去氢甲基睾丸素(大力补,dianabol)是蛋白同化激素的一种.Herbert(1984)报告服用此类药物的运动员曾出现SGOT、SGPT或LDH值的升高.滥用者甚至可发生紫癜性肝病或肝癌.然而从形态方面研究其毒性的实验资料甚少.本文介绍长期连续服用大力补小鼠肝脏的超微结构的改变.C_(57)BL小鼠36只,雄性,日龄约90天.大力补系本所合成室制备.用2%的淀粉糊调匀,经口灌喂.按药量0、1、10mg/只·天将动物分为3组,每组9只.每周喂药5次(周2～6).连续给药5、9及13周后第2天,每组活杀3只,立即切取肝脏3块.经3.1%戊二醛和1%锇酸两次固定,乙醇梯度脱水,环氧树酯GE-100包埋,超薄切片.用枸掾酸铅-醋酸铀染色.用Philips400-T电子显微镜观察.其余9只小鼠未给予任何处理,作为正
Dehydro-methyltestosterone (dianabol) is a protein anabolic hormone, and Herbert (1984) reported that SGOT, SGPT, or LDH values have been elevated in athletes taking these drugs. Abusers may even develop purpuric Liver disease or liver cancer.However, there are few experimental data on the morphological studies of its toxicity.This paper describes the ultrastructural changes of the liver in rats after vigorous administration for a long time.Among 36 male C57BL mice, Day vigorously fill the Department of the synthesis room prepared with 2% starch paste and mix thoroughly, oral feeding according to the dose of 0,1,10 mg / only · day the animals were divided into 3 groups of 9. Weekly Drugs 5 times (week 2 ~ 6) .Continuous administration of 5,9 and 13 weeks after the first two days, each group killed 3, immediately cut the liver 3. After the 3.1% glutaraldehyde and 1% osmium tetroxide two Fixed with ethanol gradient dehydration, embedded in epoxy resin GE-100, ultra-thin sections.Cleared with lead citrate-uranyl acetate.All the other 9 mice were treated with no treatment, as positive