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根据McClure偶联酶反应理论,通过实验对偶联酶法检验L-天冬酰胺酶活力的反应条件进行了动力学分析,确定了合理的附加酶用量,建立了偶联酶法检验此酶活力程序。用该程序测定的底物K_m值和产物的抑制常数K_1值与文献值相符。
According to the McClure coupling reaction theory, the kinetic analysis of the reaction conditions of L-asparaginase activity was carried out through experiments and the reasonable amount of additional enzyme was determined. A coupled enzyme assay was used to test the activity of L-asparaginase. . The K_m value of the substrate measured by the program and the K_1 value of the inhibition constant of the product are in accordance with the literatures.