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目的 研究重组人干扰素α2b、α1b、β1b和ω1b对严重急性呼吸综合征 (SARS)病毒在细胞培养上的抑制作用。方法 采用细胞病变抑制法在人横纹肌瘤 (Rda)细胞系培养上测定干扰素的抗SARS病毒活性。结果 高度纯化的重组人干扰素α2b、α1b、β1b和ω1b在Rda细胞培养上抑制SARS病毒产生 5 0 %细胞病变 (CPE)的最小活性单位各为 (16 0 5± 12 9 5 )IU ml、(14 9 0± 71 7)IU ml、(6 9 5± 6 1 5 )IU ml、(87 3± 4 7 1)IU ml或各为 (0 6± 0 5 )ng ml、(10 6± 5 1)ng ml、(3 5± 3 1)ng ml、(0 9± 0 5 )ng ml。结论 本研究中所用的重组人干扰素在细胞培养上均具有较好的抑制SARS病毒的活性。本研究就各型干扰素抗病毒敏感性的差异及干扰素预防SARS病毒感染潜在的临床应用价值进行了讨论
Objective To study the inhibitory effect of recombinant human interferon α2b, α1b, β1b and ω1b on the cell culture of severe acute respiratory syndrome (SARS) virus. Methods The anti-SARS virus activity of interferon was determined on the culture of human rhabdomyosarcoma (Rda) cell line by cytopathic effect inhibition. Results The minimal active units of highly purified recombinant human interferon α2b, α1b, β1b and ω1b in inhibition of SARS virus-producing 50% cytopathic effect (CPE) in cultured Rda cells were (165 ± 125) IU ml, (14 9 ± 71 7) IU ml, (6 95 ± 6 1 5) IU ml, (87 3 ± 4 7 1) IU ml or (0 6 ± 0 5) ng ml each, 5 1) ng ml, (3 5 ± 3 1) ng ml, (0 9 ± 0 5) ng ml. Conclusion The recombinant human interferon used in this study has better activity of inhibiting SARS virus in cell culture. This study discusses the differences in the antiviral susceptibility of various types of interferon and the potential clinical value of interferon in preventing SARS virus infection