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目的 观察诱导型一氧化氮合酶 (iNOS)在红藻氨酸(KA)癫痫大鼠海马内的表达及L 精氨酸 (L Arg)和L 硝基精氨酸 (L NNA)慢性干预的影响。方法 采用惊厥剂量的KA(1 0mg·kg- 1 )诱导大鼠癫痫发作 ,以NOS抑制剂L NNA(50mg·kg- 1 )和NO前体L Arg(40mg·kg- 1 )进行干预 ,对大鼠的癫痫发作行为及KA后不同时间点的海马内i NOSmRNA ,通过RT PCR观察其表达。结果 KA可使动物发生时间相关性癫痫发作 ,L NNA预处理后使KA诱导的癫痫发作明显加重 ,而L Arg预处理后使KA诱导的癫痫发作减弱。iNOSmRNA在KA处理后 3h开始有微弱的表达 ,且随着时间的延长逐渐增加 ,2 4h达到最高水平 ,2d及3d时未见表达 ,但 7d时又出现高表达 ;经L NNA预处理的动物 ,KA后 1h其海马结构中未出现iNOSmRNA ,但L Arg预处理后再给予KA后 1h ,可见微弱的iNOSmRNA表达。结论 红藻氨酸给药后一定时间 ,癫痫大鼠海马结构中可出现iNOSmRNA表达 ,L Arg慢性干预也有一定影响
Objective To observe the expression of inducible nitric oxide synthase (iNOS) in the hippocampus of kainic acid (KA) in epileptic rats and L-arginine (L Arg) and nitro-L-arginine (L NNA) chronic intervention influences. Methods The seizures were induced by convulsant dose of KA (10 mg · kg -1) in rats. Interfered with NOS inhibitor L NNA (50 mg · kg -1) and NO precursor L Arg (40 mg · kg -1) The seizure behavior of rats and iNOS mRNA in hippocampus at different time points after KA were observed by RT PCR. Results KA can cause the time-related seizures in animals. L-NNA pretreatment significantly aggravated the KA-induced seizures, while KA-induced seizures decreased after L Arg preconditioning. iNOS mRNA began to weaken at 3h after KA treatment, and gradually increased with time, reaching the highest level at 24 h, no expression at 2 d and 3 d, but high expression at 7 d; The iNOS mRNA did not appear in the hippocampus 1 h after KA, but weak iNOS mRNA was seen 1 h after L Arg pretreatment. Conclusions At certain time after the administration of kainic acid, iNOS mRNA expression may appear in the hippocampus of epileptic rats, and the chronic intervention of L Arg also has a certain effect