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目的:基于组织异体移植技术,研究将正常SD(Sprague-Dawley)大鼠阴茎海绵体组织和盆神经节移植到Nu/Nu裸鼠肾被膜下的可行性。方法:无菌条件下获取SD大鼠阴茎组织和盆神经节,在手术显微镜下将上述组织移植到Nu/Nu裸鼠肾被膜下,分别于移植后1周和4周检测移植物的组织病理学变化及移植物内部细胞增殖情况。结果:移植1周后,移植到Nu/Nu裸鼠肾被膜下的阴茎海绵体组织与正常阴茎海绵体组织形态一致,在阴茎海绵体窦内充满血液;移植4周后,靠近肾的阴茎海绵体组织接近正常组织形态,而远离肾的阴茎海绵体组织表现为纤维化,仍可在阴茎海绵体窦观察到血液。盆神经节移植后1周,移植物内部组织结构与正常盆神经节相近,可见多个“细胞团”样结构存在,在靠近肾的部位有新生血管样结构形成;移植后4周,在远离肾的盆神经节组织内观察到血管存在,“细胞团”样结构仍较明显。此外,移植后4周,移植到Nu/Nu裸鼠肾被膜下的海绵体组织和盆神经节中可见ki67阳性的细胞存在,提示移植物内部分细胞仍存在增殖活性。结论:将正常SD大鼠阴茎海绵体组织和盆神经节移植到裸鼠肾被膜下后,至少可以存活4周,移植物内部组织结构与正常SD大鼠阴茎海绵体和盆神经节组织结构基本一致,可能与肾被膜下提供了移植物所需的局部微环境及移植物内部逐渐形成血液循环有关。
OBJECTIVE: To investigate the feasibility of transplanting corpus cavernosum and pelvic ganglion in normal SD rats (Sprague-Dawley rats) into the renal capsule of Nu / Nu nude mice based on tissue allotransplantation. METHODS: The penile tissue and pelvic ganglion of SD rats were obtained under aseptic conditions. The above tissues were transplanted under the renal capsule of Nu / Nu nude mice under the operation microscope. The histopathological changes of the grafts were examined at 1 week and 4 weeks after transplantation Neo-Confucianism and cell proliferation in the graft. RESULTS: One week after transplantation, the corpus cavernosum transplanted into the kidney capsule of Nu / Nu nude mice was consistent with the morphology of normal corpus cavernosum and filled with blood in the corpus cavernosum. After 4 weeks of transplantation, the penis sponge near the kidney Body tissue is near normal tissue, whereas corpus cavernosum far from the kidney is fibrotic and blood is still visible at the penis cavernous sinus. One week after pelvic ganglion transplantation, the internal organization of the graft was similar to that of the normal pelvic ganglion, showing multiple “cell clusters” -like structures with neovascular-like structures near the kidney. Four weeks after transplantation, Vasculature was observed in the pelvic ganglia tissue far from the kidney, and the “cell mass” -like structure was still evident. In addition, ki67-positive cells were found in cavernous tissues and pelvic ganglia that were transplanted into the renal capsule of Nu / Nu nude mice 4 weeks after transplantation, suggesting that some of the intracellular grafts still have proliferative activity. CONCLUSION: After transplanted into corpora cavernosum in nude mice, the corpus cavernosum and pelvic ganglion of normal SD rats can survive for at least 4 weeks. The internal organization of the grafts and the histological structure of the corpus cavernosum and pelvic ganglion in normal SD rats Consistency may be related to the subrenal capsule providing the local microenvironment required for the graft and the gradual blood circulation within the graft.