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目的观察在不同剂量蜕皮甾酮(ecdysterone,EDS)作用下,体外培养的神经干细胞(neuralstemcells,NSCs)增殖、分化情况。方法原代培养新生SD大鼠海马NSCs,经EDS处理后,传代细胞克隆形成率、四甲基偶氮唑盐比色法观察神经干细胞增殖能力的变化,免疫荧光细胞染色技术、流式细胞术检测神经干细胞分化情况。结果与对照组相比,中低剂量组(50、100、200mg/L),对大鼠海马NSCs增殖能力无显著影响,而高剂量的EDS(400mg/L及800mg/L)干预组则明显抑制了NSCs的增殖。在分化条件下,EDS200mg/L干预组显著提高NSCs向神经元分化的比例,其余各组无统计学差异。结论EDS能够调节体外培养的大鼠海马NSCs的增殖和分化水平,在合适的剂量下明显提高了神经元的分化比例。
Objective To observe the proliferation and differentiation of neural stem cells (NSCs) cultured in vitro with different doses of ecdysterone (EDS). Methods Primary cultured neonatal Sprague-Dawley rats hippocampus NSCs were treated with EDS, the colony formation rate of passaged cells and MTT assay were used to observe the change of neural stem cell proliferation ability, immunofluorescence staining technique, flow cytometry. Detect neural stem cell differentiation. Results Compared with the control group, the low-dose group (50, 100, 200 mg/L) had no significant effect on the proliferation of rat hippocampal NSCs, but the high-dose EDS (400 mg/L and 800 mg/L) intervention group was significantly Inhibition of proliferation of NSCs. Under differentiation conditions, EDS200mg/L intervention group significantly increased the proportion of NSCs neuron differentiation, and the other groups had no statistical difference. Conclusion EDS can regulate the proliferation and differentiation of rat hippocampal NSCs cultured in vitro, and significantly increase the proportion of neuron differentiation at appropriate doses.