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Background Pulmonary surfactant dysfunction may contribute to the development of ventilator induced lunginjury(VILI).Tracheal gas insuffiation(TGI)is a technique in which fresh gas is introduced into the trachea andaugment ventilation by reducing the dead space of ventilatory system,reducing ventilatory pressures and tidalvolume(V_T)while maintaining constant partial arterial CO_2 pressure(PaCO_2).We hypothesised that TGI limitedpeak inspiratory pressure(PIP)and V_T and would minimize conventional mechanical ventilation(CMV)inducedpulmonary surfactant dysfunction and thereby attenuate VILI in rabbits with acute lung injury(ALI).Methods ALI was induced by intratracheal administration of lipopolysaccharide in anaesthetized,ventilatedhealthy adult rabbits randomly assigned to continuous TGI at 0.5 L/min(TGI group)or CMV group(n=8 foreach group),and subsequently ventilated with limited PIP and V_T to maintain PaCO_2 within 35 to 45 mmHg for 4hours.Physiological dead space to V_T ratio(V_D/V_T),dynamic respiratory compliance(Cdyn)and partial arterialO_2 pressure(PaO_2)were monitored.After ventilation,lungs were analysed for total phospholipids(TPL),totalproteins(TP),pulmonary surfactant small to large aggregates ratio(SA/LA)in bronchoalveolar lavage fluid(BALF)and for determination of alveolar volume density(V_v),myeloperoxidase and interleukin(IL)-8.Results TGI resulted in significant(P<0.05 or P<0.01)decrease in PIP[(22.4±1.8)cmH_2O vs (29.5±1.1)cmH_2O],V_T[(6.9±1.3)ml/kg vs(9.8±1.11)ml/kg],V_D/V_T[(32±5)% VS(46±2)%],TP[(109±22)mg/kg vs(187±25)mg/kg],SA/LA(2.5±0.4 vs 5.4±0.7),myeloperoxidase[(6.2±0.5)U/g tissue vs(12.3±0.8)U/g tissue]and IL-8[(987±106)ng/g tissue vs(24±3) mN/m]of BALF,and significant(P<0.05)increase in Cdyn[(0.47±0.02)ml·cmH_2O~(-1)·kg~(-1) vs(0.31±0.02)ml·cmH_2O~(-1)·kg~(-1)],PaO_2[(175±24)mmHg vs (135±26)mmHg],TPL/TP(52±8 vs 33±11) and Vv(0.65±0.05 vs 0.44±0.07)as compared with CMV.Conclusions In this animal model of ALI,TGI decreased ventilatory requirements(PIP,V_T and V_D/V_T),resulted in more favourable alveolar pulmonary surfactant composition and function and less severity of lunginjury than CMV.TGI in combination with pressure limited ventilation may be a lung protective strategy for ALI.
Background Pulmonary surfactant dysfunction may contribute to the development of ventilator induced lung in jury (VILI). Tracheal gas insuffiation (TGI) is a technique in which fresh gas is introduced into the trachea andaugment ventilation by reducing the dead space of ventilatory system, reducing ventilatory pressures and While hypothalysed that TGI limitedpeak inspiratory pressure (PIP) and V_T and would minimize conventional mechanical ventilation (CMV) induced pulmonary tubular dysfunction and therefore attenuated VILI in rabbits with acute lung injury (PaCO_2) ALI was induced by intratracheal administration of lipopolysaccharide in anaesthetized, ventilated healthy adult rabbits randomly assigned to continuous TGI at 0.5 L / min (TGI group) or CMV group (n = 8 foreach group), and subsequently ventilated with limited PIP and V_T to maintain PaCO_2 within 35 to 45 mmHg for 4 hours. Physiological dead space to V_T ratio (V_D / After ventilation, lungs were analyzed for total phospholipids (TPL), total proteins (TP), pulmonary surfactant small to large aggregates ratio (SA / LA) in bronchoalveolar lavage fluid (BALF) and for determination of alveolar volume density (V_v), myeloperoxidase and interleukin (IL) -8.Results TGI resulted in significant decrease in PIP [(22.4 ± 1.8) cmH_2O vs 29.5 ± 1.1 cmH 2 O], V_T [(6.9 ± 1.3) ml / kg vs (9.8 ± 1.11) ml / kg], V_D / V_T [(32 ± 5)% VS (46 ± 2)%] (6.2 ± 0.5) U / g tissue vs (12.3 ± 0.8) vs (109 ± 22) mg / kg vs 187 ± 25 mg / (987 ± 106) ng / g tissue vs (24 ± 3) mN / m] of BALF, and significant (P <0.05) increase in Cdyn [(0.47 ± 0.02) ml · PaO_2 [(175 ± 24) mmHg vs (135 ± 26) cmH 2 O -1 · kg -1 vs 0.31 ± 0.02 ml · cmH 2 O -1 · kg -1] mmHg], TPL / TP (52 ± 8 vs 33 ± 11) and Vv (0.65 ± 0.05 vs 0.44 ± 0.07) as compared with CMV.Conclusions In this animal model of ALI, TGI decreased ventilatory requirements (PIP, V_T and V_D / V_T) resulted in more favourable alveolar pulmonary surfactant composition and function and less severity of lung in jury than CMV.TGI in combination with pressure limited ventilation may be a lung protective strategy for ALI.