论文部分内容阅读
为了研究种植转基因大豆在土壤生态系统中对固氮细菌的影响,应用PCR-DGGE技术分析种植不同大豆处理下土壤细菌固氮酶nifH基因的分子多样性。结果表明:低GC含量的厚壁菌门出现在DGGE图谱的上部,而且受生育时期影响比较明显;绿菌门相对保守,不受生育时期和种植品种的影响;在测序得到的46个结果中,共计28个属于变形菌门,占60.8%,广泛分布于DGGE胶的各个部位,大部分较稳定,不受种植品种或者采样时间的影响。相对于不同生育时期对根际土壤固氮细菌的影响,种植转基因大豆、亲本非转基因大豆和普通大豆之间的差异并不明显,表明种植转基因大豆对土壤固氮细菌没有明显的影响。
In order to study the effect of planting transgenic soybeans on nitrogen-fixing bacteria in soil ecosystem, the molecular diversity of soil bacterial nitrogenase nifH gene was analyzed by PCR-DGGE. The results showed that the low-GC content of FBS appeared in the upper part of the DGGE map and was more affected by the fertility period. The chlorophyta was relatively conservative and was not affected by the growth period and the cultivars. Among the 46 sequences , A total of 28 belong to the proteobacteria, accounting for 60.8%, widely distributed in various parts of the gel DGGE, most of the more stable, not affected by the species or sampling time. Compared with the effects of different growth stages on rhizosphere soil nitrogen-fixing bacteria, the difference between planting transgenic soybean, parental non-transgenic soybean and ordinary soybean was not obvious, indicating that planting transgenic soybean had no obvious effect on soil nitrogen-fixing bacteria.