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以建立一个筛选抗血栓药物的体内微血栓模型为主要目的,本实验采用显微操作仪和微量注射技术,将二磷酸腺苷(ADP)溶液(1×10~(-2)M,5×10~(-2)M)微量注射到大鼠软脑膜微血管表面,在微血管中定位、定量诱发出白色的血小板性附壁血栓。通过对血栓形成过程的定量分析,观察几种体外血小板聚集抑制剂(阿斯匹林、654-2、川芎嗪)对血栓形成的影响。结果表明:在不损伤血管壁的条件下,单纯ADP(2μl/次)对微静脉的血栓诱发率达85%,病理切片结果发现血栓体主要由血小板构成。三种血小板聚集抑制剂均表现有不同程度的抗血栓作用。以上结果提示该微血栓模型在研究抗血栓药物及血栓形成机制方面可能有一定的实用价值。
In order to establish an in-vivo microthrombosis model for screening antithrombotic drugs, this experiment uses a micromanipulator and microinjection technique to apply adenosine diphosphate (ADP) solution (1×10-2 M, 5×). 10 ~ (-2) M) microinjection into the surface of rat pial microvessels, localization and quantification of white platelet-derived mural thrombus in microvessels. Through the quantitative analysis of the thrombosis process, the effect of several in vitro platelet aggregation inhibitors (aspirin, 654-2, tetramethylpyrazine) on thrombosis was observed. The results showed that the thrombosis-inducing rate of AVP (2 μl/times) to venous plexus reached 85% without damaging the blood vessel wall. Pathological sections showed that the thrombus was mainly composed of platelets. All three platelet aggregation inhibitors showed different degrees of antithrombotic effects. The above results suggest that the microthrombosis model may have some practical value in the study of antithrombotic drugs and thrombosis mechanism.