论文部分内容阅读
MADS-box基因编码多种具有重要生物学功能的转录调节因子,能够激活或抑制其他基因的转录,在调节植物根、叶、花和果实的发育尤其在开花植物花的发育过程中起到重要的调控作用。将东北白桦树中克隆得到的BpMADS3基因导入玉米中,验证其在玉米中的功能。结果表明,利用In-Fushion技术成功构建了BpMADS3基因的单子叶植物表达载体pTF101.1-T-nos-Ubi-BpMADS3,采用花粉管通道法将该基因导入玉米自交系合344中,获得5个T2代PCR和RT-PCR阳性株系,转基因株系H1-25和H1-76的散粉期和抽丝期均比对照提前,穗重、穗长、穗粗和百粒重等产量相关性状显著或极显著高于对照。研究结果初步证明,BpMADS3基因的导入对玉米花期提前和产量增加有一定的功效。
MADS-box gene encodes a variety of transcriptional regulatory factors with important biological functions, which can activate or inhibit the transcription of other genes and plays an important role in regulating the development of plant roots, leaves, flowers and fruits, especially in the flowering process of flowering plants The regulatory role. The BpMADS3 gene cloned from the birch of Northeast China was introduced into maize to verify its function in maize. The results showed that the recombinant plasmid pTF101.1-T-nos-Ubi-BbiMADS3 with the BpMADS3 gene was successfully constructed by In-Fushion technology and introduced into maize inbred line 344 by pollen tube pathway to obtain 5 A total of T2 generation PCR and RT-PCR positive strains, the transgenic lines H1-25 and H1-76 at the time of powdering and spinning were all higher than those of the control in terms of yield, panicle weight, panicle length, panicle weight and 100-grain weight Significantly or significantly higher than the control. The preliminary results of the research proves that the introduction of BpMADS3 gene has certain effect on the advance of popcorn and the increase of yield.