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目的:研究磁性聚乳酸羟基乙酸氧化酚砷纳米微粒对人肝癌细胞生长的抑制作用。方法:运用超声乳化-溶剂挥发法制备磁性聚乳酸羟基乙酸氧化酚砷纳米微粒;透射电镜观察纳米微球形态;MTT法检测纳米微粒对肝癌细胞SMMC-7721生长的抑制作用;流式细胞术检测细胞凋亡。结果:磁性聚乳酸氧化酚砷纳米微粒外观呈规则球型,其粒径尺寸平均为290nm。磁性纳米微粒抑制人肝癌细胞增殖,且具有一定的时间和浓度依赖性。1.0μmol/L纳米微粒组作用24h肝癌细胞生长的抑制率为(4.6±0.9)%,作用48h抑制率为(11.4±1.2)%。流式细胞术检测可见肝癌细胞凋亡峰出现,细胞周期阻滞在S+G2/M期。结论:磁性聚乳酸羟基乙酸氧化酚砷纳米微粒具有抑制肝癌细胞SMMC-7721增殖的作用,处于G0/G1期的SMMC-7721细胞可能为其药物作用的目标。
OBJECTIVE: To study the inhibitory effect of magnetic poly (lactic-co-glycolic acid) oxidized phenolic arsenic nanoparticles on the growth of human hepatoma cells. Methods: Magnetic poly (lactic-co-glycolic acid) oxidized phenolic arsenic nanoparticles were prepared by ultrasonic emulsification-solvent evaporation method. Morphology of nanospheres was observed by transmission electron microscopy. The inhibitory effect of nanoparticles on SMMC-7721 hepatoma cells was detected by MTT assay. Flow cytometry Apoptosis. Results: The appearance of magnetic polylactic acid oxidized phenolates nanoparticles was regular spherical with an average particle size of 290 nm. Magnetic nanoparticles inhibit the proliferation of human hepatoma cells, and have a certain time and concentration-dependent. The inhibitory rate of 1.0μmol / L nanoparticle group for 24h hepatoma cell growth was (4.6 ± 0.9)%, and the inhibitory rate 48h was (11.4 ± 1.2)%. Flow cytometry showed that hepatocellular carcinoma cell apoptosis peak appears, cell cycle arrest in the S G2 / M phase. CONCLUSION: Magnetic poly (DL-lactic-co-glycolic acid) oxidized phenolic arsenic nanoparticles can inhibit the proliferation of SMMC-7721 hepatoma cells. SMMC-7721 cells in G0 / G1 phase may be the target of their drug action.