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Metamizole sodium was chosen as a representative of unstable analytes for investigation by discussing the effects of oxygen and solvent on its degradation reaction using the capillary electrophoresis technique.A possible degradation mechanism was deduced from the observed behavior and was confirmed by infra-red spectroscopic study.The degradation reaction could be inhibited obviously by methanol instead of water as the solvent of analyte.Under the optimized conditions:separation voltage of 20 kV,and 5 mmol/L disodium hydrogen phosphate and 5 mmol/L borax with 10% methanol(pH 9.12)as the running buffer,the standard curve of metamizole sodium was linear in a range of 3.77—74.07 mg/L.A satisfactory result was achieved when the technique was used to detect metamizole sodium in tablet.
Metamizole sodium was chosen as a representative of unstable analytes for investigation by discussing the effects of oxygen and solvent on its degradation reaction using the capillary electrophoresis technique. A possible degradation mechanism was deduced from the observed behavior and was confirmed by infra-red spectroscopic study. The degradation reaction could be obviously evident by methanol instead of water as the solvent of analyte. Under the optimized conditions: separation voltage of 20 kV, and 5 mmol / L disodium hydrogen phosphate and 5 mmol / L borax with 10% methanol (pH 9.12 ) as the running buffer, the standard curve of metamizole sodium was linear in a range of 3.77-74.07 mg / LA satisfactory result was achieved when the technique was used to detect metamizole sodium in tablet.