论文部分内容阅读
韩国民间用药中,常使用三百草(Saururus chinensis)的地上部分治疗水肿、黄疸和淋病。为寻找具有肝脏解毒作用的化合物,本文以四氯化碳致毒的大鼠原代培养的肝细胞作为筛选系统,观察该药物的细胞保护作用。 实验材料和方法:实验药材从汉城国立大学药学院药植园采集,经甲醇等有机溶剂提取、层析,分别得到化合物Ⅰ(槲皮素-3-O-β-D-葡糖醛酸吡喃糖甙)和化合物Ⅱ(槲皮素-3-O-D-葡糖醛酸吡喃糖苷甲酯)。经冷冻
In folk medicine in South Korea, the aerial parts of Saururus chinensis are commonly used to treat edema, jaundice and gonorrhea. In order to find the compound with hepatic detoxification, we used the primary cultured hepatocytes induced by carbon tetrachloride as a screening system to observe the cytoprotective effect of this drug. Experimental Materials and Methods: The experimental herbs were collected from the pharmacy plantation of Seoul National University and extracted with methanol and other organic solvents to obtain Compound I (quercetin -3-O-β-D-glucuronopyridine Glycoside) and compound II (quercetin-3-OD-glucuronide methyl ester). Freeze