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目的:研究丹参酮ⅡA对无血清培养诱致PC12细胞凋亡的抑制作用。方法:以噻唑兰(MIT)法测定PC12细胞存活率;用流式细胞术检测DNA含量及凋亡细胞百分率;DNA琼脂糖电泳法观察DNA断裂。结果:去血清培养(12h)可诱导PC12细胞凋亡(细胞凋亡率96.07％)。丹参酮Ⅱ A(0.1,1μmol·L~(-1))显著降低凋亡细胞百分率(细胞凋亡率分别为25.71％和4.89％),并减少DNA断裂。丹参酮ⅡA(0.01-10μmol·L~(-1))抑制氰化钠(20mmol·L~(-1))、谷氨酸钠(0.5mmol·L~(-1))和硝普钠(0.5mmol·L~(-1))所致的细胞毒性。结论:丹参酮Ⅱ A可抑制去血清培养引起的PC12细胞凋亡。
Objective: To study the inhibitory effect of tanshinone II A on the apoptosis of PC12 cells induced by serum-free culture. Methods: The survival rate of PC12 cells was measured by thiazolyl (MIT) method. DNA content and percentage of apoptotic cells were detected by flow cytometry. DNA fragmentation was observed by DNA agarose gel electrophoresis. RESULTS: Serum-deprived cultured (12 h) could induce apoptosis of PC12 cells (apoptosis rate was 96.07%). Tanshinone II A (0.1,1 μmol·L-1) significantly reduced the percentage of apoptotic cells (apoptosis rate was 25.71% and 4.89%, respectively) and reduced DNA fragmentation. Tanshinone IIA (0.01-10 μmol·L -1) inhibits sodium cyanide (20 mmol·L -1), sodium glutamate (0.5 mmol·L -1), and sodium nitroprusside (0.5 The cytotoxicity caused by mmol·L~(-1)). Conclusion: Tanshinone II A can inhibit apoptosis of PC12 cells induced by serum-free culture.