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为研究丰香草莓种子辐射诱变后培育的植株性状遗传变异程度,本研究用3种剂量梯度的γ射线(~(60)Co)对丰香草莓的种子进行辐射诱变处理,并筛选合适的ISSR引物进行辐射效果及辐射后群体的遗传多样性分析。结果表明:(1)筛选出的4个ISSR引物可用于草莓辐照选育后的遗传变异分析;4条引物共扩增出31条条带,其中24条为多态性条带,多态性比例达79.14%。(2)3个辐照群体的遗传相似均在96%以上,高于与未辐射处理对照的平均遗传相似系数,辐照群体间的基因多样度、分化指数以及分子方差变异分析结果均表明遗传变异主要是由辐照引起的。(3)对遗传相似系数进行聚类分析表明辐照群体产生了较大的遗传变异;Shannon’s信息指数及Nei’s遗传多样性指数分析结果显示高剂量辐照的遗传多样性也相对高。
In order to study the degree of genetic variation of plant traits cultivated in Fengxiang strawberry seed by radiation, three kinds of dose gradient γ-rays (~ (60) Co) were used to mutate the seeds of Fengxiang strawberry, The ISSR primers were used to analyze the effect of radiation and the genetic diversity of the population after radiation. The results showed that: (1) Four ISSR primers were selected for genetic variation analysis after strawberry irradiation. A total of 31 bands were amplified by four primers, of which 24 were polymorphic bands. Polymorphism Sex ratio of 79.14%. (2) The genetic similarity of the three irradiation groups was above 96%, higher than the average genetic similarity coefficient with the control of non-radiation treatment, the genetic diversity among irradiation groups, the differentiation index and the variance analysis of molecular variance showed that genetic Variation is mainly caused by irradiation. (3) The clustering analysis of genetic similarity coefficient showed that the genetic variation of irradiation population was larger; Shannon’s information index and Nei’s genetic diversity index analysis showed that the genetic diversity of high dose radiation was relatively high.